Products

Anti-EDB Immunocytokine, scFv (L19)-IFNγ (ACFP-SH084)

This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-EDB scFv to IFNγ. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the EDB as well as the biological activity of IFNγ. The scFv (L19) directed against the EDB domain of fibronectin was fused to the N-terminus of a cysteine-free mutant of mouse IFN-γ. The resulting fusion protein was capable of targeting new blood vessels in solid tumors, and the targeting efficiency was strikingly increased in tumor-bearing knockout mice lacking the interferon-γ receptor. ScFv (L19)-interferon-γ displayed a strong antitumor effect in both subcutaneous and metastatic murine F9 teratocarcinomas, but was not efficacious as single agent when used to treat C51 and CT26 tumors. This immunocytokine was designed for treating Advanced solid tumors, Advanced pancreatic cancer, Metastatic melanoma.      

Specifications
Target
EDB
Molecule Class
scFv-IFNγ
Species Reactivity
Human
Host
Human
Expression Host
CHO
Clone
L19
Related Disease
Advanced solid tumors, Advanced pancreatic cancer, Metastatic melanoma
Cytokines 1
IFNγ
Cytokines 1 Description
Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class ofinterferons. The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulated with phytohemagglutinin, and by others as a product of antigen-stimulated lymphocytes or tuberculin-sensitized mouse peritoneal lymphocytes challenged with PPD; the resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. Those reports also contained the basic observation underlying the now widely employed interferon gamma release assay used to test for tuberculosis. In humans, the IFNγ protein is encoded by the IFNG gene.
Cytokine 1 Species
Mouse
Applications
This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
Alternative Names
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS
Entrez Gene ID
2335
UniProt ID
P02751

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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