This product is a recombinant antibody that recognizes ERBB2. The monoclonal antibody B1D2 reacts with the epitope of ERBB2.
Figure 1 High Her2 expression is required for efficient targeted exosome uptake.
Parental HEK293, and HEK-Her2 overexpressing cell lines were incubated with exosomes as described above, followed by staining with WGA-Alexafluor555 (red) and Hoechst (blue) post-fixation. Images were acquired on the Opera high-throughput confocal microscope.
Longatti, A., Schindler, C., Collinson, A., Jenkinson, L., Matthews, C., Fitzpatrick, L., ... & Tigue, N. (2018). High affinity single-chain variable fragments are specific and versatile targeting motifs for extracellular vesicles. Nanoscale, 10(29), 14230-14244.
Figure 2 High affinity anti-Her2 targeted exosomes are required for efficient uptake into Her2 positive tumour cell lines.
The indicated cell lines were incubated with targeted (G98A, ML39 and B1D2) and irrelevant scFv CFSE-exosomes in duplicate for 4 h, and cells were analysed for CFSE-exosome uptake by flow cytometry.
Longatti, A., Schindler, C., Collinson, A., Jenkinson, L., Matthews, C., Fitzpatrick, L., ... & Tigue, N. (2018). High affinity single-chain variable fragments are specific and versatile targeting motifs for extracellular vesicles. Nanoscale, 10(29), 14230-14244.
Figure 3 Non-targeted and anti-Her2-scFv-exosomes can be generated via genetic manipulation of HEK293 cells.
Binding of recombinant Her2-Fc to immobilised exosomes by ELISA; irrelevant scFv (open circles), G98A scFv (squares), ML39 scFv (triangles) and B1D2 (closed circles). Error bars represent the SEM of three independent experiments where samples were analysed in duplicate.
Longatti, A., Schindler, C., Collinson, A., Jenkinson, L., Matthews, C., Fitzpatrick, L., ... & Tigue, N. (2018). High affinity single-chain variable fragments are specific and versatile targeting motifs for extracellular vesicles. Nanoscale, 10(29), 14230-14244.
Figure 4 Two-color immunofluorescence of anti-ErbB2 receptor-grafted peripheral blood T cells.
Nontransduced peripheral blood T cells and T cells grafted with anti-ErbB2 immunoreceptors with different affinities of the binding domain (no. 1–5) were simultaneously incubated with a PE-conjugated anti-CD3 mAb and an FITC-conjugated anti-human IgG1 Ab and analyzed by flow cytometry.
Chmielewski, M., Hombach, A., Heuser, C., Adams, G. P., & Abken, H. (2004). T cell activation by antibody-like immunoreceptors: increase in affinity of the single-chain fragment domain above threshold does not increase T cell activation against antigen-positive target cells but decreases selectivity. The Journal of Immunology, 173(12), 7647-7653.
Figure 5 Activation of anti-ErbB2 receptor-grafted T cells by solid phase bound Ag.
Serial dilutions of recombinant ErbB2-Fc fusion protein (A) or of an anti-human IgG-Fc Ab (B) were coated onto microtiter plates. Peripheral blood T cells were grafted with anti-ErbB2 immunoreceptors with different Kd values of the anti-ErbB2 scFv domain and incubated for 48 h (1 × 10⁴ receptor-grafted T cells/well). IFN-γ in the supernatant was recorded by ELISA.
Chmielewski, M., Hombach, A., Heuser, C., Adams, G. P., & Abken, H. (2004). T cell activation by antibody-like immunoreceptors: increase in affinity of the single-chain fragment domain above threshold does not increase T cell activation against antigen-positive target cells but decreases selectivity. The Journal of Immunology, 173(12), 7647-7653.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-005 | Anti-Human ErbB2 Recombinant Antibody (Trastuzumab) | FC, IP, ELISA, Neut, FuncS, IF, IHC | IgG1 - kappa |
TAB-033CT | Human Anti-ERBB2 Recombinant Antibody (TAB-033CT) | ELISA, WB | Human IgG1, κ |
TAB-038CT | Human Anti-ERBB2 Recombinant Antibody (TAB-038CT) | FuncS, FC | Humanized IgG1 |
TAB-039CT | Anti-Human HER2/neu Recombinant Antibody (h1E11) | WB | Humanized antibody |
TAB-041CT | Human Anti-ERBB2 Recombinant Antibody (TAB-041CT) | SPR, ELISA, WB | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-761 | Anti-ERBB2 Recombinant Antibody (Margetuximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
TAB-049CT | Anti-Human HER2/neu Recombinant Antibody (ChA21) | IHC, Inhib, WB, Activ, FC | Chimeric antibody (mouse/human) |
TAB-049CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
TAB-049CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G022E | Anti-ERBB2 immunotoxin 4D5 (scFv)-PE | Cytotoxicity assay, Function study | |
AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
AGTO-G022S | Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap | Cytotoxicity assay, Function study |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-126 | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (PFBL-126) | ELISA, WB, FuncS | Human Fab |
PFBC-089 | Mouse Anti-ERBB2 Recombinant Antibody (clone A21); Fab Fragment | ELISA, WB, FC | Mouse Fab |
PFBL-463 | Human Anti-ERBB2 Recombinant Antibody (clone mAb37); Fab Fragment | WB | Human Fab |
PSBW-161 | Mouse Anti-ERBB2 Recombinant Antibody (clone chA21); scFv Fragment | FC, WB | Mouse scFv |
HPAB-M0510-YC-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-M0510-YC-F(E)) | FC, FuncS | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PNBL-066 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-066) | SPR | Llama VHH |
PNBL-067 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-067) | SPR | Llama VHH |
PNBL-069 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-069) | ELISA | Llama VHH |
TAB-032CT | Anti-Human HER2/neu Therapeutic Single Domain Antibody | WB | Single domain antibody |
TAB-046CT | Anti-Human HER2/neu Therapeutic Single Domain Antibody (TAB-046CT) | ELISA, Inhibition, FC | Single domain antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-034CT | Anti-Human HER2/neu Recombinant Antibody (pertuzumab ) | WB | |
TAB-036CT | Anti-Human HER2/neu Recombinant Antibody (6B2) | ELISA, WB | |
TAB-036CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (6B2) | ELISA, WB | |
TAB-037CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (7C2/7F3) | ELISA, WB | |
PABX-082-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr6) | WB, ELISA, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-055CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment | WB | Human antibody |
TAB-056CT-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-056CT-S(P)) | WB | Human scFv |
PABX-081-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr3) | WB, ELISA, FuncS | scFv |
PABX-081-F (E) | Recombinant Human Anti-HER2 Antibody Fab Fragment (Gr3 ) | WB, ELISA, FuncS | Fab |
PABX-082-F (E) | Recombinant Human Anti-HER2 Antibody Fab Fragment (Gr6) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
Gly-039LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-118LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Mouse antibody |
Gly-145LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Gly-148LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0103MZ | Recombinant Mouse Anti-ERBB2 Antibody (clone C980M) | ELISA | Mouse antibody |
MOB-014LC | Mouse Anti-ERBB2 Recombinant Antibody (MOB-014LC) | ELISA, FC | Mouse IgG |
MOB-014LC-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (MOB-014LC-F(E)) | ELISA, FC | Mouse Fab |
MOB-014LC-S(P) | Mouse Anti-ERBB2 Recombinant Antibody; scFv Fragment (MOB-014LC-S(P)) | ELISA, FC | Mouse scFv |
FAMAB-0098YC | Mouse Anti-ERBB2 Recombinant Antibody (clone E402) | Cyt | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
BRD-0695MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
BRD-0779MZ | Chicken Anti-ErbB2 Polyclonal IgY | ICC, IF, IHC, IP | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Timigutuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Pertuzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Margetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Trastuzumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0679YY-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone H2-18); scFv Fragment | ELISA | Human scFv |
FAMAB-0171JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone C6L1); scFv Fragment | ELISA, FuncS | Human scFv |
FAMAB-0172JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone C6H2); scFv Fragment | ELISA, FuncS | Human scFv |
FAMAB-0173JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone C6H211); scFv Fragment | ELISA, FuncS | Human scFv |
PSBS-0070 | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (PSBS-0070) | ELISA, FuncS | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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