Rabbit Anti-F3 Recombinant Antibody (clone 8B10) (CAT#: ZG-0661J)

This product is a rabbit antibody that recognizes F3.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Rabbit Anti-F3 Antibody (ZG-0661J) in WB

Figure 1 Rabbit Anti-F3 Antibody (ZG-0661J) in WB

Western Blot
Positive WB detected in: HL60 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate, NTERA-2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, Rat brain tissue
All lanes: F3 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 28 kDa
Observed band size: 46 kDa

IHC

Figure 2 Rabbit Anti-F3 Antibody (ZG-0661J) in IHC

Figure 2 Rabbit Anti-F3 Antibody (ZG-0661J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • A synthesized peptide derived from human Tissue Factor
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human F3
  • Species Reactivity
  • Human, Rat
  • Clone
  • 8B10
  • Applications
  • ELISA, WB, IHC
  • Conjugate
  • Uconjugated

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
    WB:1:500-1:5000, IHC:1:50-1:200

Target

  • Alternative Names
  • Coagulation Factor III, Tissue Factor; Coagulation Factor III (Thromboplastin, Tissue Factor); Tissue Factor; TF; Coagulation Factor III; Thromboplastin; CD142 Antigen; CD142; TFA; CNTO-859; CNTO 859

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "F3"

Deglycosylated Antibody (Non-glycosylated IgGs)

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Rabbit Monoclonal Antibody

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MOR-1226 Hi-Affi™ Rabbit Anti-F3 Recombinant Antibody (clone DS1226AB) WB, IHC-P, ICC, IF Rabbit IgG

Human Antibody

CAT Product Name Application Type
TAB-473CQ Anti-Human F3 Recombinant Antibody (Tisotumab) ELISA, IHC, FC, IP, IF, Inhib IgG1, κ

Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-0573CQ Mouse Anti-F3 Recombinant Antibody (clone hATR-5) ELISA, Inhib Mouse IgG4

Recombinant Antibody

CAT Product Name Application Type
HPAB-0172CQ Human Anti-F3 Recombinant Antibody (HPAB-0172CQ) ELISA, FC, ADCC Chimeric (mouse/human) IgG1
HPAB-0173CQ Human Anti-F3 Recombinant Antibody (HPAB-0173CQ) ELISA, FC, ADCC Chimeric (mouse/human) IgG1, λ
HPAB-0174CQ Human Anti-F3 Recombinant Antibody (HPAB-0174CQ) ELISA, FC, ADCC Chimeric (mouse/human) IgG1, λ
HPAB-0175CQ Human Anti-F3 Recombinant Antibody (HPAB-0175CQ) ELISA, WB, Inhib Humanized IgG1, κ
PABC-450 Mouse Anti-F3 Recombinant Antibody (clone 10H10) ELISA, FuncS Mouse IgG1, κ

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-473CQ Afuco™ Anti-F3 ADCC Recombinant Antibody (Tisotumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, Inhib ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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