Rabbit Anti-APC Recombinant Antibody (clone R08-6J7) (CAT#: VS3-XY3261)
It is a recombinant rabbit antibody specific for APC (APC regulator of WNT signaling pathway), which acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It specifically targets human and rat APC. This product has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13362/1401_C6_5_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13362/1401_C6_5_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_B_9_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_B_9_5_selected.jpg
Figure 3 Cerebral cortex
(Neuropil
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_B_9_5.jpg
(Neuropil
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_B_9_5.jpg
Figure 4 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_9_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_7_4.jpg
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_7_4.jpg
Figure 6 Kidney
(Collecting ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_7_5.jpg
(Collecting ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_7_5.jpg
Figure 7 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_6_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134982-APC
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134982-APC
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat APC
- Species Reactivity
- Human, Rat
- Clone
- R08-6J7
- Applications
- WB, IHC-P, ICC, IF, IP
- Related Disease
- Familial Adenomatous Polyposis 1; Gastric Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46
- Gene ID
- 324
- UniProt ID
- P25054
- Long Name
- APC regulator of WNT signaling pathway
- Sequence Similarities
- Belongs to the adenomatous polyposis coli (APC) family.
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Microtubule
- Post Translation Modifications
- Phosphorylated by GSK3B.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
- Protein Refseq
- NP_000029.2; NP_001120982.1; NP_001120983.2
- Function
- Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Pancreatic Cancer
Prostate Cancer
Throid Cancer
Hepatocellular Carcinoma
Cushing Syndrome
Canonical Wnt Signaling Pathway
JAK-STAT Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-72-S(P) | Recombinant Anti-human APC Antibody scFv Fragment | IP, IF, Biosensors, FuncS | scFv |
| MHH-72-S(P) | Recombinant Human Anti-human APC Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| HPAB-0095-WJ-S(P) | Human Anti-APC Recombinant Antibody; scFv Fragment (HPAB-0095-WJ-S(P)) | ELISA | Humanized scFv |
| HPAB-J0036-YC-S(P) | Mouse Anti-aPC Recombinant Antibody; scFv Fragment (HPAB-J0036-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-J0037-YC-S(P) | Mouse Anti-aPC Recombinant Antibody; scFv Fragment (HPAB-J0037-YC-S(P)) | ELISA | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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