CBGlyco™ Glycoprotein Staining Kit (CAT#: Glyco-047CL)

Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.


Specific Inquiry
  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 750 mL
  • Target Molecule
  • Proteins (Glycoproteins)
  • Label or Dye
  • Fuchsin Sulfite
  • Detection Method
  • Colorimetric
  • Detection Location
  • In-Gel Detection, In-Blot Detection
  • Contents & storage
  • Sufficient For: 10 mini gels (SDS-PAGE)
    • Oxidation Reagent, 2.5 g powder to make 250 mL
    • Reduction Reagent, 1.25 g powder to make 250 mL
    • Positive Control (Horseradish Peroxidase), 1 mg
    • Negative Control (Soybean Trypsin Inhibitor), 1 mg
    • Glycoprotein Stain, 250 mL

    Store all components at 4°C.

Background

  • Description
  • The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

    To use the Glycoprotein Staining Kit, simply treat the gel or membrane containing separated proteins treated with the provided periodate-based Oxidation Reagent. This treatment oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are reacted with the Glycoprotein Stain Reagent to form Schiff-base bonds that result in the production of magenta bands of the stain. Traditionally called the periodate-acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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