CBGlyco™ Hydrazide-PEG4-Biotin (Glyco-027CL)

Hydrazide-PEG4-Biotin (also called Biotin-PEG4-Hydrazide) is an aldehyde-reactive biotinylation reagent that contains a 4-unit polyethylene glycol (PEG) spacer arm for increased hydrophilicity.      

Product Size
50 mg
Label or Dye
Pegylated, Extra-Long
DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Cell Permeability
Chemical Reactivity
Carboxylic Acid, Ketone, Aldehyde
Contents & storage
Store at 4°C.
Biotin-PEG4-Hydrazide enables biotinylation of glycoproteins and other molecules containing aldehydes or oxidizable sugar groups. The hydrazide group reacts spontaneously with aldehyde or ketone groups to form a stable hydrazone bond. Aldehyde groups can be created by gentle periodate oxidation of cis-diols in sialic acid and other sugars in carbohydrates. The hydrophilic, 4-unit polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG segment also adds length and flexibility to the spacer arm, minimizing steric hindrance involved with binding to avidin molecules.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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