CBGlyco™ Reversible Protein Stain Kit for Nitrocellulose Membranes (CAT#: Glyco-052CL)

Reversible Protein Stain Kit for Nitrocellulose Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on nitrocellulose membrane after transfer from polyacrylamide gels.

  • Datasheet
  • MSDS
  • COA

Specifications

  • Product Size
  • 1.5 L
  • Target Molecule
  • Protein
  • Label or Dye
  • Proprietary Mix
  • Detection Method
  • Colorimetric
  • Detection Location
  • In-Blot Detection
  • Contents & storage
  • Sufficient For: 10 nitrocellulose membranes (8 cm x 8 cm) from mini gels (SDS-PAGE)
    • Reversible Stain, 250 mL
    • Destain, 2 x 500 mL
    • Stain Eraser, 250 mL

    Store at room temperature

Background

  • Description
  • This kit for membrane staining uses a nondestructive, reversible, reliable and sensitive method to stain and detect proteins on nitrocellulose membranes. The kit is a superior alternative to Ponceau S stains for evaluating the efficiency of protein transfer following SDS-PAGE and before immunoblotting. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The staining protocol is simple, quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed.

    The Reversible Protein Stain Kit for Nitrocellulose Membranes has excellent avidity for a broad range of proteins, resulting in low protein-to-protein variability and enabling most proteins to be detected at very low levels (25 to 50ng per band). Membrane Stain is superior to other protein stains available for nitrocellulose membranes. For example, Ponceau S is less sensitive and results in red bands that easily fade and are difficult to photograph. Coomassie dye is a sensitive stain, but it permanently binds to proteins and can interfere with western blotting.

    The kit uses a dye that has a high affinity for protein but does not permanently bind. The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents, and the technique is compatible with general western blot systems. The treated membrane does not interfere with conventional chemiluminescent or chromogenic detection using HRP and alkaline phosphatase substrates. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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