Recombinant Anti-CDPK1 VHH Single Domain Antibody (1B7) (CAT#: PNBL-035)

Anti-CDPK1 VHH Single Domain Antibody is a recombinant protein produced in E. coli.

  • Published Data
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 1B7 recognizes a calcium-dependent conformation of TgCDPK1

IRDye800-labeled 1B7 was used to probe decreasing concentrations of total T. gondii lysate or recombinant kinase (Top).Samples were separately probed for parasite actin (ACT1; red) or the 6xHis-tag on recombinant proteins (HIS; green).

Ingram, J. R., Knockenhauer, K. E., Markus, B. M., Mandelbaum, J., Ramek, A., Shan, Y., ... & Lourido, S. (2015). Allosteric activation of apicomplexan calcium-dependent protein kinases. Proceedings of the National Academy of Sciences, 112(36), E4975-E4984.

WB

Figure 2 1B7 recognizes a calcium-dependent conformation of TgCDPK1

Incubation of parasite lysates with immobilized 1B7 depletes TgCDPK1/TgCDPK3 in the presence of CaCl2 but not in EGTA. The immunoprecipitated material could be eluted from the beads by subsequent incubation with EGTA. Blot was probed with 1B7 (green), and ACT1 (red) is included as a control.

Ingram, J. R., Knockenhauer, K. E., Markus, B. M., Mandelbaum, J., Ramek, A., Shan, Y., ... & Lourido, S. (2015). Allosteric activation of apicomplexan calcium-dependent protein kinases. Proceedings of the National Academy of Sciences, 112(36), E4975-E4984.

IP

Figure 3 1B7 recognizes a calcium-dependent conformation of TgCDPK1

IP of recombinant kinases by 1B7 in the presence of 1 mM CaCl2 or EGTA. The domain structure of each kinase, indicating the KD and each of the EF hands (I–IV), is depicted for wild-type (WT), D368A/D415A (N-lobe mutant), and D451A/D485A (C-lobe mutant).

Ingram, J. R., Knockenhauer, K. E., Markus, B. M., Mandelbaum, J., Ramek, A., Shan, Y., ... & Lourido, S. (2015). Allosteric activation of apicomplexan calcium-dependent protein kinases. Proceedings of the National Academy of Sciences, 112(36), E4975-E4984.

Figure 4 1B7 recognizes a calcium-dependent conformation of TgCDPK1

Calcium dependency of TgCDPK1 activation or 1B7 binding. Values are normalized to maximal activity or binding.

Ingram, J. R., Knockenhauer, K. E., Markus, B. M., Mandelbaum, J., Ramek, A., Shan, Y., ... & Lourido, S. (2015). Allosteric activation of apicomplexan calcium-dependent protein kinases. Proceedings of the National Academy of Sciences, 112(36), E4975-E4984.

Inhib

Figure 5 1B7 inhibits TgCDPK1 and related kinases.

In vitro kinase assay with increasing concentrations of 1B7.

Ingram, J. R., Knockenhauer, K. E., Markus, B. M., Mandelbaum, J., Ramek, A., Shan, Y., ... & Lourido, S. (2015). Allosteric activation of apicomplexan calcium-dependent protein kinases. Proceedings of the National Academy of Sciences, 112(36), E4975-E4984.


Specifications

  • Host Species
  • Llama
  • Derivation
  • Llama
  • Type
  • Llama VHH
  • Species Reactivity
  • T. gondii
  • Clone
  • 1B7
  • Applications
  • IP, ELISA, WB, Inhib

Applications

  • Application Notes
  • The antibody was validated for Immunoprecipitation, Western Blot, Inhibition. For details, refer to Published Data.

Target

  • Alternative Names
  • ATCDPK1; CALCIUM-DEPENDENT PROTEIN KINASE; calcium-dependent protein kinase 1; CDPK1; CPK10; F6A14.1; F6A14_1

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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