Mouse Anti-Meta I Rhodopsin Recombinant Antibody (clone K42-41L) (CAT#: PABW-086)

Figure 1 ELISA demonstrating that the K42-41L antibody recognizes the original antigen, opsin (open diamonds), and dark-adapted rhodopsin in native retinal rod disk membranes (open squares) with essentially equal affinity.

The dark-adapted or fully light-bleached membranes were bound to ELISA plates, washed, and presented with a dilution series of antibody K42-41L. Bound antibody was detected as described in the text.

Piscitelli, C. L., Angel, T. E., Bailey, B. W., Hargrave, P., Dratz, E. A., & Lawrence, C. M. (2006). Equilibrium between metarhodopsin-I and metarhodopsin-II is dependent on the conformation of the third cytoplasmic loop. Journal of Biological Chemistry, 281(10), 6813-6825.

Figure 2 K42-41L inhibits formation of metarhodopsin-II.

A and B demonstrate photolysis of rhodopsin upon a series of light flashes that bleach 8% of the total rhodopsin per flash. Three consecutive flash intervals are shown, and the arrows indicate the time points at which flashes were provided. The square symbols represent metarhodopsin-II stabilized in the absence of antibody K42-41L, while circlesrepresent samples incubated with antibody K42-41L. A, metarhodopsin-II stabilized at pH 8 by the addition of the Ac-VLEDLRS(Abu)GLF peptide, a high affinity analog of the C-terminal tail of the transducin alpha subunit. B, metarhodopsin-II stabilized at pH 6.7, as described under "Experimental Procedures."

Piscitelli, C. L., Angel, T. E., Bailey, B. W., Hargrave, P., Dratz, E. A., & Lawrence, C. M. (2006). Equilibrium between metarhodopsin-I and metarhodopsin-II is dependent on the conformation of the third cytoplasmic loop. Journal of Biological Chemistry, 281(10), 6813-6825.