Human Anti-A. fumigatus cell walls Recombinant Antibody (clone 8A1) (CAT#: FAMAB-1576CQ)

Figure 1 mAbs show distinct localization patterns.

A. fumigatus 237 was grown for 12-14 h, fixed, incubated with primary mAb, followed by FITC-conjugated anti-mouse secondary antibody, Calcofluor White to label chitin and Hoechst 33258 to label nuclei. Primary antibodies: mAb1E2 (a), mAb8A1 (b) and mAb19H1 (c). Left column, FITC; right column, chitinous cell wall and nuclei. Arrows mark conidia; arrowheads mark septa; the double arrowhead marks a newly formed branch from main hypha.

Momany, M., Lindsey, R., Hill, T. W., Richardson, E. A., Momany, C., Pedreira, M., ... & Roberts, K. A. (2004). The Aspergillus fumigatus cell wall is organized in domains that are remodelled during polarity establishment. Microbiology, 150(10), 3261-3268.

Figure 2 Basal and apical wall domains do not overlap.

(a, b) A. fumigatus 237 was grown for 6 h, fixed and labelled with mAb8A1 (green) and mAb16C4 (red). Left column, differential interference contrast (DIC); middle column, mAb 8A1 followed by FITC-conjugated secondary antibody; right column, mAb 16C4 directly coupled to Alexa Fluor 546.

Momany, M., Lindsey, R., Hill, T. W., Richardson, E. A., Momany, C., Pedreira, M., ... & Roberts, K. A. (2004). The Aspergillus fumigatus cell wall is organized in domains that are remodelled during polarity establishment. Microbiology, 150(10), 3261-3268.

Figure 3 mAbs 8A1 and 16C4 recognize wall epitopes.

A. fumigatus germlings were plasmolysed in 2·67 M sorbitol before labelling with mAbs and visualization with FITC-conjugated anti-mouse secondary antibody. DIC and fluorescence images of germling labelled by mAb8A1 (a, b) or mAb16C4 (c, d). Bar, 5 μm.

Momany, M., Lindsey, R., Hill, T. W., Richardson, E. A., Momany, C., Pedreira, M., ... & Roberts, K. A. (2004). The Aspergillus fumigatus cell wall is organized in domains that are remodelled during polarity establishment. Microbiology, 150(10), 3261-3268.