Mouse Anti-Azobenzenearsonate Recombinant Antibody (clone 36-60) (CAT#: FAMAB-0006CQ)
This product is a recombinant mouse antibody clone 36-60, which specifically binds to ABA (Azobenzenearsonate).
Specific Inquiry
Southern blot
Figure 1 Mouse Anti-Azobenzenearsonate Antibody (clone 36-60) in Southern blot.
Southern blot analysis of VH rearrangement in Id36-60-bearing hybridoma DNAs. DNAs from the indicated hybridomas were digested with EcoRI (A) or HindlIl (B), electrophoresed, blotted, and hybridized with the 3' JH probe. HindlIl-cleaved λ phage DNA fragments were size markers. Weak bands in A are denoted by carats. The germ-line and rearranged VH36-60 bands (2.4 and 1.1 kb, respectively) are noted in B. The BALB/c hybridomas Max 4.4 and 15.4 no longer secrete Id36-60 and also show an absence of rearranged VH36-60.
Near, R. I., Juszczak, E. C., Huang, S. Y., Sicari, S. A., Margolies, M. N., & Gefter, M. L. (1984). Expression and rearrangement of homologous immunoglobulin VH genes in two mouse strains. Proceedings of the National Academy of Sciences, 81(7), 2167-2171.
Southern blot
Figure 2 Mouse Anti-Azobenzenearsonate Antibody (clone 36-60) in Southern blot.
Polymorphisms in the VH36_60 gene family. Kidney DNA from the indicated strains was digested with HindlIl (A) or EcoRI (B) and analyzed by Southern blots using the VH36_60 probe. Markers are 5'-labeled HindIII-cleaved phage A DNA fragments. An additional band of 1.3 kb is present in blots of A/J and BALB/c EcoRI DNA digests when hybridization was strong.
Near, R. I., Juszczak, E. C., Huang, S. Y., Sicari, S. A., Margolies, M. N., & Gefter, M. L. (1984). Expression and rearrangement of homologous immunoglobulin VH genes in two mouse strains. Proceedings of the National Academy of Sciences, 81(7), 2167-2171.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- ABA (Azobenzenearsonate)
- Clone
- 36-60
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Azobenzenearsonate; ABA
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0006CQ-F(E) | Mouse Anti-Azobenzenearsonate Recombinant Antibody (clone 36-60); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0006CQ-S(P) | Mouse Anti-Azobenzenearsonate Recombinant Antibody (clone 36-60); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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