Mouse Anti-FCGR2 Recombinant Antibody (clone AT130-5) (CAT#: FAMAB-1624CQ)

Figure 1 Cross-blocking of anti-FcγRIIB mAb.

BALB/c splenocytes were incubated with unlabelled mAb before labelling with FITC-mAb and PE-CD19 (to identify B cells) and analysed by flow cytometry. OKT3 was used as an irrelevant control (Irr). AT130-2 and AT130-5 binding could only be blocked by themselves and each other, whereas the binding of AT128 and 2.4G2 could be blocked by all of the anti-FcγRIIB mAb.

Williams, E. L., Tutt, A. L., French, R. R., Chan, H. C., Lau, B., Penfold, C. A., ... & Cragg, M. S. (2012). Development and characterisation of monoclonal antibodies specific for the murine inhibitory F cγ RIIB (CD 32 B). European journal of immunology, 42(8), 2109-2120.

Figure 2 Cross-blocking of anti-FcγRIIB mAb.

BALB/c splenocytes were incubated with unlabelled mAb IgG (top panel) or F(ab')2 (bottom panel) before the addition of rabbit-anti-ova/ova-FITC ICs) and analysed by flow cytometry. Data in (A) and (B) represent an example of three experiments, where single samples were run per condition.

Williams, E. L., Tutt, A. L., French, R. R., Chan, H. C., Lau, B., Penfold, C. A., ... & Cragg, M. S. (2012). Development and characterisation of monoclonal antibodies specific for the murine inhibitory F cγ RIIB (CD 32 B). European journal of immunology, 42(8), 2109-2120.

Figure 3 Binding profiles of anti-FcγRIIB mAb.

BALB/c peripheral blood lymphocytes from WT, FcγRIIB−/− or FcγRIII−/− mice labelled with FITC-mAb and analysed by flow cytometry.

Williams, E. L., Tutt, A. L., French, R. R., Chan, H. C., Lau, B., Penfold, C. A., ... & Cragg, M. S. (2012). Development and characterisation of monoclonal antibodies specific for the murine inhibitory F cγ RIIB (CD 32 B). European journal of immunology, 42(8), 2109-2120.

Figure 4 Activation of FcγRIIB and SHIP-1 by anti-FcγRIIB mAb.

πBCL1 cells at varying concentrations (2, 0.4, and 0.1 × 106 cells/mL) were incubated with anti-FcγRIIB mAb and anti-idiotype mAb at 37°C for 5 min and then lysed and analysed for pFcγRIIB. The relative cell densities achieved at these cell concentrations were demonstrated by bright field microscopy, ×10 magnification (scale bar is equal to 10 μm).

Williams, E. L., Tutt, A. L., French, R. R., Chan, H. C., Lau, B., Penfold, C. A., ... & Cragg, M. S. (2012). Development and characterisation of monoclonal antibodies specific for the murine inhibitory F cγ RIIB (CD 32 B). European journal of immunology, 42(8), 2109-2120.