Human Anti-KSHV gH Recombinant Antibody (clone YC15) (CAT#: FAMAB-1346CQ)

Figure 1 Flow cytometry analysis of the YC15-PE38 immunotoxin binding to KSHV gH-expressing transfectant cell line.

YC15-PE38 and the control immunotoxin BL22-PE38 were labeled with Alexa fluor 488 and incubated at the indicated concentrations with the YC-293-gH/gL stable transfectant cell line or parental cells. After washing, the cellbound immunotoxins were detected by flow cytometry.

Cai, Y., & Berger, E. A. (2011). An immunotoxin targeting the gH glycoprotein of KSHV for selective killing of cells in the lytic phase of infection. Antiviral research, 90(3), 143-150.

Figure 2 Effect of YC15-PE38 on infectious KSHV production from activated Vero-219 cells.

Vero-219 cells were activated by recombinant baculovirus BacK50 plus sodium butyrate, and treated with different concentration of YC15-PE38 for 3 days.

Cai, Y., & Berger, E. A. (2011). An immunotoxin targeting the gH glycoprotein of KSHV for selective killing of cells in the lytic phase of infection. Antiviral research, 90(3), 143-150.

Figure 3 Direct cytotoxicity assay of YC15-PE38 against cell line expressing KSHV gH.

293 gH/gL stable transfectant cells (designated 293-gH/gL) or parental 293 cells were treated for 3 days with the indicated concentrations of YC15-PE38 or the negative control proteins YC15-PE38E553D or BL22-PE38. Cell viability was measured by the WST-8 assay as described in Section 2. Data points indicate the means of triplicate samples; error bars represent standard deviations.

Cai, Y., & Berger, E. A. (2011). An immunotoxin targeting the gH glycoprotein of KSHV for selective killing of cells in the lytic phase of infection. Antiviral research, 90(3), 143-150.