Human Anti-Malignant tumor cell Recombinant Antibody (clone 4G12) (CAT#: FAMAB-0053WJ)
This product is a human antibody with broad reactivity to malignant tumor cells, especially for lung squamous cell carcinomas, and recognizes a new tumor-associated and differentiation antigen.
Specific Inquiry
ELISA
Figure 1 Human Anti-Malignant tumor cell Antibody (clone 4G12) in ELISA.
Cell-binding reactivity of 4G12 MAbs on various lung carcinoma cell lines. Reactivity was determined by ELJSA as described in the Materials and Methods section. Target cells used were PC 10 squamous cell carcinoma (•), PC14 adenocarcinoma (A), PC13 large cell carcinoma (•), PC6 small cell carcinoma (A), and normal adult fibroblast (O). Absorbance of negative control as P3U1 culture supernatant and purified human IgM is <0.1.
Saito, H., Uchiyama, K., Nakamura, I., Hiraoka, H., Yamaguchi, Y., & Taniguchi, M. (1988). Characterization of a Human Monoclonal Antibody With Broad Reactivity to Malignant Tumor Cells1. JNCI: Journal of the National Cancer Institute, 80(10), 728-734.
SDS-PAGE
Figure 2 Human Anti-Malignant tumor cell Antibody (clone 4G12) in SDS-PAGE.
SDS-PAGE analysis of 4G12 MAbs. Human serum IgG Ganes A and D), 4G12 (lanes B and E), and human serum IgM (lanes C and F) were analyzed in SDS-PAGE under nonreduccd (lanes A, B, and C) and reduced Ganes D, E, and F) conditions. Left lane indicates molecular markers. KD = kilodaltons. See Materials and Methods section.
Saito, H., Uchiyama, K., Nakamura, I., Hiraoka, H., Yamaguchi, Y., & Taniguchi, M. (1988). Characterization of a Human Monoclonal Antibody With Broad Reactivity to Malignant Tumor Cells1. JNCI: Journal of the National Cancer Institute, 80(10), 728-734.
IHC
Figure 3 Human Anti-Malignant tumor cell Antibody (clone 4G12) in IHC.
Immunoperoxidase staining of various tumor tissues with 4G12 MAbs. A. Well-differentiated squamous cell carcinoma of lung. Note that tumor cells are strongly stained. X 60. B. Poorly differentiated squamous cell carcinoma of lung. Note heterogeneous but sometimes intense staining in some tumor cells. X 100. C. Adenoid cystic carcinoma of lung. Note that spme tumor cells are strongly stained. X 60. D. Signet ring cell type of gastric cancer. Note homogeneous staining pattern of tumor cells and no staining of infiltrated lymphocytes. X 60.
Saito, H., Uchiyama, K., Nakamura, I., Hiraoka, H., Yamaguchi, Y., & Taniguchi, M. (1988). Characterization of a Human Monoclonal Antibody With Broad Reactivity to Malignant Tumor Cells1. JNCI: Journal of the National Cancer Institute, 80(10), 728-734.
SDS-PAGE
Figure 4 Human Anti-Malignant tumor cell Antibody (clone 4G12) in SDS-PAGE.
SDS-PAGE analysis of immunoprccipitates with 4G12 panes A and C) and purified normal human IgM (lanes B and D) with Nonidet P40 extracts of radioiodinated PC 10 cells. Samples were either reduced with 2-mercaptoethanol (lanes C and D) or nonrcduced (lanes A and B). KD = kilodaltons.
Saito, H., Uchiyama, K., Nakamura, I., Hiraoka, H., Yamaguchi, Y., & Taniguchi, M. (1988). Characterization of a Human Monoclonal Antibody With Broad Reactivity to Malignant Tumor Cells1. JNCI: Journal of the National Cancer Institute, 80(10), 728-734.
FC
Figure 5 Human Anti-Malignant tumor cell Antibody (clone 4G12) in FC.
Cell-cycle-dependent expression of 4G12 antigen on PC 10 lung squamous cell carcinoma. PC 10 cells (5 X 103 ) were stained with 4G12 MAbs [thick line) and control IgM (thin line) followed by reaction with FITC-labeled anti-human IgM and then were analyzed by FACS IV. Cells negative or positive for 4G12 expression were then separated by FACS IV with a logarithmic amplifier, cloned, and cultured for 45 days.
Saito, H., Uchiyama, K., Nakamura, I., Hiraoka, H., Yamaguchi, Y., & Taniguchi, M. (1988). Characterization of a Human Monoclonal Antibody With Broad Reactivity to Malignant Tumor Cells1. JNCI: Journal of the National Cancer Institute, 80(10), 728-734.
Specifications
- Host Species
- Human
- Type
- Human IgM, λ
- Specificity
- Malignant tumor cells
- Species Reactivity
- Human
- Clone
- 4G12
- Applications
- WB
- Related Disease
- Tumors
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Tumor; Malignant tumor cell
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0053WJ-F(E) | Human Anti-Malignant tumor cell Recombinant Antibody (clone 4G12); Fab Fragment | WB | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0053WJ-S(P) | Human Anti-Malignant tumor cell Recombinant Antibody (clone 4G12); scFv Fragment | WB | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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