Mouse Anti-Migis-α Recombinant Antibody (clone 8G7) (CAT#: FAMAB-0228CQ)

Figure 1 A graph which depicts an ELISA showing the reactivity of an anti-IgA.

Fc mAb (3C10) and two anti-migis-α mAbs (8G7 and 29C11) with human IgA and constructs, which contain various human membrane-bound α chain, or other components.

Figure 2 Epitope mapping of anti-migis-α mAbs in ELISA.

A shows binding reactivity of anti-migis-α mAbs with different parts of migis-αL. B shows binding reactivity of anti-migis-α mAbs with short peptides of the N-terminal part of migis-αL.

Figure 3 Determination of the relative affinity of 8G7 and 29C11 in binding to ma in ELISA.

A shows binding strength of 8G7 and 29C11 against mα1.FcL-456S-LZ proteins. B. shows relative ability of 8G7 and 29C11 in competing with 200 nM biotin-conjugated 29C11 in binding to FcL-456S-LZ protein.

Figure 4 Staining profiles of anti-migis-α mAbs on DAKIKI cells and mα1.Fc-expressing Ramos transfectomas.

The gray profiles are negative control mAbs of the same IgG isotypes.

Figure 5 The absence of reactivity of 8G7 mAb with 8 non-mIgA-expressing cell lines.

Figure 6 The reactivity of anti-migis-α mAbs toward DAKIKI cells with or without MβCD treatments.

Figure 7 Results indicating that 3C10 and 8G7 can induce apoptosis in two mα1.Fc-expressing Ramos transfectomas, whereas 29C11 fails to do so.

Figure 8 Competition of mAbs 7C3 and 9F5 with 29C11 for binding to migis-α.

Biotin-conjugated 29C11 was mixed with various concentrations of mAbs before the mixtures were placed in microtiter wells coated with mFcL-LZ. Following incubation, HRP-conjugated avidin and its substrate, TMB, were applied for colorimetric measurements.

Hung, A. F. H., Chen, J. B., Lu, C. S., Chen, N. Y., Yu, H. M., & Chang, T. W. (2011). Lipid rafts hinder binding of antibodies to the extracellular segment of the membrane-anchor peptide of mIgA. Molecular immunology, 48(15-16), 1975-1982.