Recombinant Mouse Anti-NS1 Antibody (6D4B10) (CAT#: HPAB-N0195-YC)
The antibody thereof may specifically bind Dengue virus (DENV) serotype 4 and include: a heavy chain variable region that comprises at least one CDR amino acid sequence selected from the group consisting of: SGYNWH, YIH YS GGTN YNPS LKS, RTGTVPFAY, SYVMH, YLNPYNDDTKYNEKFKG, and GPPYALDY.
Specific Inquiry
ELISA
Figure 1 Serotype-specificity of monoclonal antibodies (MAbs 3H7A9, 8A6F2, 6D4B10 and 10H10B5) against DENV-4 determined by iELISA.
The bars show mean optical density (OD) at 450 nm which measures MAb reactivity to NS1s to all four DENV-4 serotypes, yellow fever virus (YFV), West Nile virus (WNV) and SUMO protein. MAbs 3H7A9, 8A6F2, and 6D4B10 represent anti-NS1 MAbs specific to DENV-4. MAb 10H10B5, which is reactive to SUMO*, represents one of the three non-specific MAbs generated from immunization of SUMO*-DENV-4 rNS1 fusion protein. The OD value for DENV-4-serotype specific MAbs against rNS1 of other flaviviruses and recombinant SUMO protein was < 0. By contrary, MAbs reactive only to SUMO* protein (10H10B5) did not show any reactivity to rNS1 to YFV and WNV
ELISA
Figure 2 DENV-4 serotype-specificity MAbs 3H7A9, 8A6F2, 6D4B10 and 10H10B5 as determined by fixed cell ELISA.
The bars showed mean optical density at 450 nm that measures MAb's reactivity to dimeric NS1 expressed on DENV1–4-infected Vero cells. Control included mock-infected Vero cells
ELISA
Figure 3 Analysis of optimal capture/detector MAb pair for development of DENV-4 serotype specific NS1 capture ELISA.
The curves represent limit of detection (LOD) curve for matched pairs: a 8A6F2 and biotinylated 6D4B10, and b 6D4B10 and biotinylated 8A6F2. The optical density (OD) values at 450 nm were obtained at various concentration of DENV-4 rNS1 with an optimal concentrations for coating antibody, 5-10 μg/ml; capture antibody, 1:2000 dilution and streptavidin-tagged horseradish peroxidase (HRP-SP), 1:2000. WNV was used as the control
Specifications
- Immunogen
- SUMO-DENV4 rNS l fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- DENV4
- Species Reactivity
- DENV4
- Clone
- 6D4B10
- Applications
- ELISA, WB
Applications
- Application Notes
- ELISA
NS1 capture ELISA was developed by utilizing two of the MAbs as a capture and detector antibody. In order to determine the optimal capture/detector pair for detection of DENV-4 NS1 antigen, each MAbs was tested either as a capture or a detector antibody. The optimal concentrations of capture and detector antibodies were determined by checkerboard titration ELISA. Microtiter plates were coated with MAb 8A6F2 (100 μl/well) diluted in bicarbonate buffer (pH 9.6) at concentration 10 μg/mL and incubated overnight at 4 °C. Next day, excess unbound capture MAb was removed and thereafter microtiter plates were blocked with 200 μl/well of 5% NFDM or 2% bovine serum albumin (BSA) (w/v). After 45 min incubation, microtiter plates washed 3X with PBST and thereafter 100 μl/ml of rNS1 antigen (0.5 μg/ml) and serum sample diluted 1:1 in PBST was added and incubated at 37 °C for 60 min. Following incubation and 3X washes, 100 μl/well of biotinylated MAb 6D4B10 diluted at 1:2000 in PBST were added and incubated for 1 h at 37 °C. After 3X washes, 100 μl/well peroxidase-conjugated Streptavidin was added and then microtiter plates were incubated at 37 °C for 30–60 min. After 5X washes, the reaction was visualized by adding 100 μl/well TMB liquid substrate and incubating at RT in the dark for 15 min. The reaction was then stopped with 100 μl/well TMB stop solution. The optical density (OD) was measured at 450 nm on ELISA ELx800 microplate reader.
Target
- Alternative Names
- Non-structural protein 1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-712 | Recombinant Anti-NS1 Antibody | ELISA, WB, IF, FuncS | IgG |
| PABL-070 | Recombinant Human Anti-DENV NS1 Antibody (22NS1) | WB, ELISA, IF, FuncS | IgG |
| FAMAB-1386CQ | Human Anti-NS1 Recombinant Antibody (clone ZKA25) | ELISA | Human IgG1 |
| FAMAB-1770CQ | Human Anti-NS1 Recombinant Antibody (clone ZKA35) | Block, ELISA | Human IgG1 |
| VS3-WK476 | Rabbit Anti-Ns1 Recombinant Antibody (clone JG37-82) | WB, ELISA | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-712-F(E) | Recombinant Anti-NS1 Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-712-F(E) | Recombinant Human Anti-NS1 Antibody Fab Fragment | WB, FC, FuncS | Fab |
| PFBL-070 | Recombinant Human Anti-DENV NS1 Antibody Fab Fragment (22NS1) | WB, ELISA, IF, FuncS | Fab |
| HPAB-N0195-YC-F(E) | Recombinant Mouse Anti-NS1 Antibody Fab Fragment (6D4B10) | ELISA, WB | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-712-S(P) | Recombinant Anti-NS1 Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| MHH-712-S(P) | Recombinant Human Anti-NS1 Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| PSBL-070 | Recombinant Human Anti-DENV NS1 Antibody scFv Fragment (22NS1) | WB, ELISA, IF, FuncS | scFv |
| HPAB-N0195-YC-S(P) | Recombinant Mouse Anti-NS1 Antibody scFv Fragment (6D4B10) | ELISA, WB | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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