Mouse Anti-PfHRP2 Recombinant Antibody (clone F1110) (CAT#: FAMAB-0653WJ)
This product is a mouse monoclonal antibody targeting Plasmodium falciparum histidine-rich protein 2. This antibody can be used in a variety of applications, such as ELISA, WB, Neut.
Specific Inquiry
WB
Figure 1 Reactivity of mAbF1546 and mAbF1110 with P. falciparum as assayed by western blots
The western blot assayed the reactivity of mAbF1546 (lane 1) and mAbF1110 (lane 2) with P. falciparum crude antigenic extracts, subjected to electrophoresis under denaturing conditions. Apparent MW (kDa) are shown on the left side of the immunoblot.
Ravaoarisoa, E., Zamanka, H., Fusai, T., Bellalou, J., Bedouelle, H., Puijalon, O., & Fandeur, T. (2010, July). Recombinant antibodies specific for the Plasmodium falciparum histidine-rich protein 2. In MAbs (Vol. 2, No. 4, pp. 416-427). Taylor & Francis.
IF
Figure 2 Reactivity of mAbF1546 and mAbF1110 with P. falciparum as assayed by indirect immunofluorescence.
The indirect immunofluorescence staining patterns for mAbF1546 (part 1) and mAbF1110 (part 2) were performed on air-dried blood stages of P. falciparum. Antigenic preparations were obtained from 3D7 parasites.
Ravaoarisoa, E., Zamanka, H., Fusai, T., Bellalou, J., Bedouelle, H., Puijalon, O., & Fandeur, T. (2010, July). Recombinant antibodies specific for the Plasmodium falciparum histidine-rich protein 2. In MAbs (Vol. 2, No. 4, pp. 416-427). Taylor & Francis.
ELISA
Figure 3 Reactivity of mAbF1546 and sandwich ELISA with mAbF1546 and mAbF1110.
Microtiter plates were coated with either mAbF1546 (A) or mAbF1110 (B) as capture antibody at a 10 µg.mL−1 in PBS, then reacted with decreasing concentrations of soluble PfHRP2 containing fractions equivalent to 500-0.25 IRBC µL−1 prepared by hypotonic lysis (SCHIZ-H20) or mild denaturation with 0.05% SDS in PBS (SCHIZ-0.05% SDS) of IRBC. Bound antigen was finally revealed using biotin labeled mAbF1546 and avidin peroxydase as indicated in the Materials and Methods section. The negative control (C neg) consists of equivalent preparations of uninfected human erythrocytes treated by 0.05% SDS and assayed in similar conditions.
Ravaoarisoa, E., Zamanka, H., Fusai, T., Bellalou, J., Bedouelle, H., Puijalon, O., & Fandeur, T. (2010, July). Recombinant antibodies specific for the Plasmodium falciparum histidine-rich protein 2. In MAbs (Vol. 2, No. 4, pp. 416-427). Taylor & Francis.
Specifications
- Immunogen
- P. falciparum asexual blood stages
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG1, κ
- Specificity
- Plasmodium falciparum histidine-rich protein 2
- Species Reactivity
- Plasmodium falciparum
- Clone
- F1110
- Applications
- ELISA, WB, Neut
- Related Disease
- Plasmodium falciparum infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Plasmodium falciparum histidine-rich proteins 2; PfHRP2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0654WJ | Mouse Anti-PfHRP2 Recombinant Antibody (clone F1546) | ELISA, WB, Neut | Mouse IgG1, κ |
| FAMAB-0655WJ | Shark Anti-PfHRP2 Recombinant Antibody (clone H8) | ELISA, WB, Neut | Shark VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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