Recombinant Rat Anti-Pfs48/45 Antibody (85RF45.1) (CAT#: PABC-512)
This product is a recombinant Rat antibody that can recognize Pfs48/45. 85RF45.1 binds to epitope I on Pfs48/45.
Specific Inquiry
WB
Figure 1 Pfs48/45@PbMSP1 schizont lysates (500 & 250 ng), P. falciparum gametocytes (Pf Gam.; 500 ng) and P. berghei WT schizont lysate (PbWT; 500 ng) were analyzed in Western blot analysis using anti-Pfs48/45 monoclonal 85RF45.1 (1:2000).
Densitometry analysis was performed on signals after probing Pfs48/45@PbMSP1 schizont lysate (500 ng) and a dilution series (50, 25, 12.5, 6 and 3 ng) of recombinant Pfs48/45 (r48/45; R0.10C) with antibody 45.1.
Othman, A. S., Lin, J. W., Franke-Fayard, B. M., Kroeze, H., van Pul, F. J., Chevalley-Maurel, S., ... & Long, C. A. (2018). Expression of full-length Plasmodium falciparum P48/45 in P. berghei blood stages: A method to express and evaluate vaccine antigens. Molecular and biochemical parasitology, 224, 44-49.
BL
Figure 2 Transmissionblocking (TB) activity correlates with the concentration of the anti-epitope antibodies.
Percentage inhibition of oocysts are related to the percentage competition of the anti-epitope I antibodies in the sera from mice after five immunizations with the rec10C (□) (15) and M-Pfs10C(♦) protein as calculated by using a fixed amount of peroxidase-conjugated mAb anti-epitope (85RF45.1) at a dilution of 1/20 of the serum.
Outchkourov, N. S., Roeffen, W., Kaan, A., Jansen, J., Luty, A., Schuiffel, D., ... & Stunnenberg, H. G. (2008). Correctly folded Pfs48/45 protein of Plasmodium falciparum elicits malaria transmission-blocking immunity in mice. Proceedings of the National Academy of Sciences, 105(11), 4301-4305.
ELISA
Figure 3 Two-site competition ELISAs.
mAb against epitope Ilb (85RF45.2b) was used to capture Pfs48/45 molecule from qametocyte extract. Percentage competition of sera from mice immunized with the M-Pfs10C protein (batch 1) was calculated by using a fixed amount of peroxidase-conjugated mAb anti-epitope l (Ep I) or anti-epitope Il (Ep Ill) at serum dilutions [ranging from 1/20 to 1/160 (5-0.62% vol/vol)].
Outchkourov, N. S., Roeffen, W., Kaan, A., Jansen, J., Luty, A., Schuiffel, D., ... & Stunnenberg, H. G. (2008). Correctly folded Pfs48/45 protein of Plasmodium falciparum elicits malaria transmission-blocking immunity in mice. Proceedings of the National Academy of Sciences, 105(11), 4301-4305.
Specifications
- Host Species
- Rat
- Type
- Rat IgG1, λ
- Specificity
- Pfs48/45
- Species Reactivity
- P. falciparum
- Clone
- 85RF45.1
- Applications
- ELISA, BL, WB
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Blocking and Western Blot. In Western Blot, the dilution of the antibody is 1:2000. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Pfs48/45
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-513 | Recombinant Human Anti-Pfs48/45 Antibody (TB31F) | ELISA, BL | Human IgG1, λ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-512 | Recombinant Rat Anti-Pfs48/45 Antibody Fab Fragment (85RF45.1) | ELISA, BL | Rat Fab |
| PFBC-513 | Recombinant Human Anti-Pfs48/45 Antibody Fab Fragment (TB31F) | ELISA, BL | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-512 | Recombinant Rat Anti-Pfs48/45 Antibody scFv Fragment (85RF45.1) | ELISA, BL | Rat scFv |
| PSBC-513 | Recombinant Human Anti-Pfs48/45 Antibody scFv Fragment (TB31F) | ELISA, BL | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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