Rabbit Anti-Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Recombinant Antibody (clone 3H12) (CAT#: ZG-0497U)
Rabbit Anti-Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Antibody (clone 3H12) displayed high specificity against the PAK3/PAK1/PAK2 protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Antibody (ZG-0497U) in WB
Western Blot
Positive WB detected in A549 whole cell lysate(treated with EGF or not)
All lanes Phospho-PAK1/PAK2/PAK3 antibody at 1.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65 KDa
Observed band size: 65 KDa
IHC
Figure 2 Rabbit Anti-Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Antibody (ZG-0497U) in IHC
IHC image of ZG-0497U diluted at 1:100 and staining in paraffin-embedded rat brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-PAK1/PAK2/PAK3 (S144+S141+S139)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-PAK1/PAK2/PAK3 (S144+S141+S139)
- Species Reactivity
- Human
- Clone
- 3H12
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Intellectual Developmental Disorder With Macrocephaly, Seizures, And Speech Delay, Gastroesophageal Junction Adenocarcinoma, Human Immunodeficiency Virus Type 1, Glucosephosphate Dehydrogenase Deficiency, Intellectual Developmental Disorder, X-Linked 30 and Non-Syndromic X-Linked Intellectual Disability.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- IDDMSSD; p65-PAK; PAKalpha; alpha-PAK; serine/threonine-protein kinase PAK 1; STE20 homolog, yeast; p21 protein (Cdc42/Rac)-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); EC 2.7.11.1; PAK65; PAKgamma; serine/threonine-protein kinase PAK 2; PAK-2; S6/H4 kinase; gamma-PAK; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p58; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; XLID30; PAK3beta; beta-PAK; serine/threonine-protein kinase PAK 3; adriamycin resistance-associated; oligophrenin-3; p21 (CDKN1A)-activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 3
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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