Recombinant Mouse Anti-SSPG1d Antibody (scFv-rSSPG1d) (CAT#: FAMAB-0278-CN)

This is a recombinant antibodies against SSPG1d, an endo-polygalacturonase secreted by Sclerotinia sclerotiorum. The antibody scFv-rSSPG1d is able to detect whole SSPG1d produced by the fungus.


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  • Published Data
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WB

Figure 1 Utility of ScFvs in Western blots.

Figure 1 Utility of ScFvs in Western blots.

Western blot detection of SSPG1d using ScFv-rSSPG1d. Lanes 1 and 2 refer to rSSPG1d and protein from S. sclerotiorum culture supernatants, respectively.

Yang, B., Yajima, W., Das, D., Suresh, M. R., & Kav, N. N. (2009). Isolation, expression and characterization of two single-chain variable fragment antibodies against an endo-polygalacturonase secreted by Sclerotinia sclerotiorum. Protein expression and purification, 64(2), 237-243.

ELISA

Figure 2 Detection of rSSPG1d in plant extracts by ELISA using ScFv-rSSPG1d.

Figure 2 Detection of rSSPG1d in plant extracts by ELISA using ScFv-rSSPG1d.

Results indicate a concentration-dependent increase in absorbance when rSSPG1d was used to spike both leaf and flower extracts. Data are presented as means ± SE of three biological replicates and SAS statistical analysis (p < 0.05) was used to identify significant differences among the samples as indicated by different letters above columns.

Yang, B., Yajima, W., Das, D., Suresh, M. R., & Kav, N. N. (2009). Isolation, expression and characterization of two single-chain variable fragment antibodies against an endo-polygalacturonase secreted by Sclerotinia sclerotiorum. Protein expression and purification, 64(2), 237-243.


Specifications

  • Immunogen
  • Purified N-terminally polyhistidine tagged recombinant SSPG1d protein
  • Host Species
  • Mouse
  • Derivation
  • scFv Phage display library
  • Type
  • Mouse IgG
  • Specificity
  • S. sclerotiorum SSPG1d
  • Species Reactivity
  • S. sclerotiorum
  • Clone
  • scFv-rSSPG1d
  • Applications
  • WB, ELISA

Product Property

  • Storage
  • Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Western Blot and Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.

Target

  • Alternative Names
  • SSPG1d

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone scFv-rSSPG1d"

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Recombinant Antibody

CAT Product Name Application Type
FAMAB-0279-CN Recombinant Mouse Anti-SSPG1d Antibody (scFv-3796) WB, ELISA Mouse IgG

scFv Fragment Antibody

Fab Fragment Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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