This is a recombinant antibodies against SSPG1d, an endo-polygalacturonase secreted by Sclerotinia sclerotiorum. The antibody scFv-rSSPG1d is able to detect whole SSPG1d produced by the fungus.
Figure 1 Utility of ScFvs in Western blots.
Western blot detection of SSPG1d using ScFv-rSSPG1d. Lanes 1 and 2 refer to rSSPG1d and protein from S. sclerotiorum culture supernatants, respectively.
Yang, B., Yajima, W., Das, D., Suresh, M. R., & Kav, N. N. (2009). Isolation, expression and characterization of two single-chain variable fragment antibodies against an endo-polygalacturonase secreted by Sclerotinia sclerotiorum. Protein expression and purification, 64(2), 237-243.
Figure 2 Detection of rSSPG1d in plant extracts by ELISA using ScFv-rSSPG1d.
Results indicate a concentration-dependent increase in absorbance when rSSPG1d was used to spike both leaf and flower extracts. Data are presented as means ± SE of three biological replicates and SAS statistical analysis (p < 0.05) was used to identify significant differences among the samples as indicated by different letters above columns.
Yang, B., Yajima, W., Das, D., Suresh, M. R., & Kav, N. N. (2009). Isolation, expression and characterization of two single-chain variable fragment antibodies against an endo-polygalacturonase secreted by Sclerotinia sclerotiorum. Protein expression and purification, 64(2), 237-243.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0279-CN | Recombinant Mouse Anti-SSPG1d Antibody (scFv-3796) | WB, ELISA | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0278-CN-S(P) | Recombinant Mouse Anti-SSPG1d Antibody scFv Fragment (scFv-rSSPG1d) | WB, ELISA, SPR | Mouse scFv |
FAMAB-0279-CN-S(P) | Recombinant Mouse Anti-SSPG1d Antibody scFv Fragment (scFv-3796) | WB, ELISA, SPR | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0278-CN-F(E) | Recombinant Mouse Anti-SSPG1d Antibody Fab Fragment (Fab-rSSPG1d) | WB, ELISA, SPR | Mouse Fab |
FAMAB-0279-CN-F(E) | Recombinant Mouse Anti-SSPG1d Antibody Fab Fragment (Fab-3796) | WB, ELISA, SPR | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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