Human Anti-ZIKV EDII Recombinant Antibody (clone G9E) (CAT#: FAMAB-1558CQ)
This product is a recombinant human antibody that recognizes ZIKV EDII. The antibody was purified by affinity chromatography. It can be used in Neut, WB, ELISA.
Specific Inquiry
Neut
Figure 1 FRNT assays against a panel of ZIKV strains and related flaviviruses were performed for G9E.
Collins, M. H., Tu, H. A., Gimblet-Ochieng, C., Liou, G. J. A., Jadi, R. S., Metz, S. W., ... & de Silva, A. M. (2019). Human antibody response to Zika targets type-specific quaternary structure epitopes. JCI insight, 4(8).
Figure 2 A panel of 6 flavivirus cross-reactive and 6 ZIKV-specific mAbs were competed with A9E and G9E in BOB assays.
Competition assays (BOB) with a panel of mAbs with known binding specificities was performed to localize the epitopes of A9E and G9E.
Collins, M. H., Tu, H. A., Gimblet-Ochieng, C., Liou, G. J. A., Jadi, R. S., Metz, S. W., ... & de Silva, A. M. (2019). Human antibody response to Zika targets type-specific quaternary structure epitopes. JCI insight, 4(8).
Figure 3 A9E and G9E epitope-binding IgGs are widely represented in polyclonal plasma following natural ZIKVinfection.
(A) Blockade of binding (BOB) against A9E and G9E was tested among plasma at a 1:20 dilution from ZIKV and DENV cases from the UNC Traveler's study, Nicaragua, and Sri Lanka, as was done for mAbs in Figure 4C. (B) The ZIKV cases were subdivided into primary (1°) and secondary (2°) ZIKV (ZIKV infection in a DENV-immune host). (C)Paired plasma specimens at 1:10 dilution from symptomatic ZIKV cases in Nicaragua were analyzed by BOB at early(day 21 after symptom onset) versus late (6 months after symptom onset) convalescence. An unpaired Student's t test was performed to determine differences in means between groups as indicated by bars. **P < 0.01; ***P < 0.001;****P < 0.0001. ns, not significant.
Collins, M. H., Tu, H. A., Gimblet-Ochieng, C., Liou, G. J. A., Jadi, R. S., Metz, S. W., ... & de Silva, A. M. (2019). Human antibody response to Zika targets type-specific quaternary structure epitopes. JCI insight, 4(8).
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- ZIKV
- Species Reactivity
- ZIKV
- Clone
- G9E
- Applications
- Neut, WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Zika virus EDII; ZIKV
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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