Recombinant Mouse Antibody (CB4-1) is capable of binding to HIV-1 p24, expressed in mammalian cells.
Figure 1 Deconvolution of the excess heat capacity functions of CB4-1 (A), Fab CB4-1 (B) and Fc CB4-1 (C).
Solid curves represent baseline corrected experimental endotherms and dashed curves show the component two-state transitions obtained by deconvolution. The transitions of CB4-1 are numbered from 1 to 5. The sums of the component transitions shown as dashed lines closely ¢t the experimental curves.
Welfle, K. , Misselwitz, R. , Hausdorf, G. , Höhne W, & Welfle, H. . (1999). Conformation, ph-induced conformational changes, and thermal unfolding of anti-p24 (hiv-1) monoclonal antibody cb4-1 and its fab and fc fragments. Biochimica Et Biophysica Acta, 1431(1), 120.
Figure 2 Dissociation Constants of CB4-1 Interacting with UmuD and Candiapepsin and Influences of CB4-1 Mutations on Peptide and Protein Recognition.
(A) Inhibition of CB4-1-p24 interaction by p24, UmuD, and candidapepsin determined by competitive ELISA. Soluble proteins compete with solid phase-adsorbed p24 for CB4-1 binding. 1/c (c = concentration of the inhibitors) is plotted versus E0/(E0-EX) (E = extinction with no inhibitor (0) and various inhibitor concentrations [x]). The slope of the fitted curves correspond to the inhibition constants (Friguet et al. 1985).
(B) BIAcore measurements of UmuD interacting with solid phase-linked CB4-1. Overlay plot of various concentrations of UmuD (50 μM, 12.5 μM, 3.1 μM, and 0.78 μM [sensograms 1–4, respectively]) captured by CB4-1-coated chips. Samples were injected at t = 0 s. Dissociation started at t = 300 s. The association rate constant kon was determined as 3.0 × 103 M-1s-1, the dissociation rate constants koff as 6.0 × 103 s-1 resulting in Kd = 2.0 × 10−6 M.
(C) CB4-1 single chain mutants VL:Phe94Ala and VH:Tyr32Ala as well as the wild-type CB4-1 single chain Fv fragment (wt) were tested for their ability to recognize immobilized peptide 1 (GATPEDLNQKLAGN) and peptide 2 (GLYEWGGARITNTD) as well as alcohol dehydrogenase (supertope 1-derived) and candidapepsin (supertope 2-derived). Binding of the CB4-1 single chains carrying the c-myc-tag were detected using the anti-c-myc antibody 9E10 and a peroxidase-conjugated anti-mouse antibody.
Kramer, A. . (1997). Molecular basis for the binding promiscuity an anti-p24 (hiv-1) monoclonal antibody. Cell, 91(6), 799-809.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0268MC | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone 406A) | WB, IF, sELISA, ELISA, RIPA | Mouse IgG1 |
MRO-912CQ | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone 1946) | ELISA, IF | Mouse IgG1 |
MRO-913CQ | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone 1-B-390) | EIA, IHC, IF, WB | Mouse IgG1 |
MRO-914CQ | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone 8416591) | ICC, ELISA | Mouse IgG1 |
MRO-915CQ | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone #24-2) | WB, IF | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-186LC | Anti-HIV-1 p24 Recombinant Antibody (6A9/5) | ELISA | Human antibody |
MRO-186LC-S(P) | Anti-HIV-1 p24 Recombinant Antibody scFv Fragment (6A9/5) | ELISA | Human antibody |
MRO-187LC-S(P) | Anti-HIV-1 p24 Recombinant Antibody scFv Fragment (6D9/4) | ELISA | Human antibody |
MRO-081LC-F(E) | Anti-HIV-1 p24 Recombinant Antibody Fab Fragment (3A6) | ELISA | Human antibody |
MRO-082LC-F(E) | Anti-HIV-1 p24 Recombinant Antibody Fab Fragment (19H2) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1613 | Rabbit Anti-HIV-1 p24 Recombinant Antibody (clone DS1613AB) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-2850CQ-F(E) | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone 1E8G2); Fab Fragment | ELISA, WB, IF | Mouse Fab |
MRO-4239CQ-F(E) | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone p3JB9); Fab Fragment | ELISA | Mouse Fab |
MRO-4240CQ-F(E) | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone p6F4); Fab Fragment | ELISA | Mouse Fab |
MRO-4241CQ-F(E) | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone 5E2.A3); Fab Fragment | ELISA | Mouse Fab |
MRO-4242CQ-F(E) | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone V7-8); Fab Fragment | ELISA, RIA, WB | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABC-580 | Recombinant Llama Anti-HIV-1 p24 Single Domain Antibody (PABC-580) | ELISA | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-S0099-YC-S(P) | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone A2); scFv Fragment | ELISA, WB | Mouse scFv |
HPAB-S0100-YC-S(P) | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone A3); scFv Fragment | ELISA, WB | Mouse scFv |
HPAB-S0101-YC-S(P) | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone A5); scFv Fragment | ELISA, WB | Mouse scFv |
HPAB-S0102-YC-S(P) | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone D1); scFv Fragment | ELISA | Mouse scFv |
HPAB-S0103-YC-S(P) | Mouse Anti-HIV-1 p24 Recombinant Antibody (clone D2); scFv Fragment | ELISA, WB | Mouse scFv |
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