Mouse Anti-TSBP Recombinant Antibody (clone S67-27) (CAT#: PABL-736)

Figure 1 Surface plasmon resonance of S67-27 binding to Kdo analogs.

Sensorgram overlays for the binding of ( A ) 12.5, 25, 50, 75, 100, 200, 400, and 800 nM S67-27 Fab to 980 RUs of immobilized Kdo(2 → 4)Kdo-BSA, the inset shows equilibrium analysis, and ( B ) 10, 20, 40, 80, 160, 320, 640 and 1280 nM S67–27 Fab to 160 RUs of immobilized Kdo(2 → 8)Kdo(2 → 4)Kdo-BSA. The open black circles are the data points and the curves the fitting of the data to a 1:1 interaction model; the inset shows equilibrium analysis. Sensorgram overlays for the binding of ( C ) 5, 10, 20, 40, 60 and 80 nM S67-27 Fab to 570 RUs of immobilized Kdo(2 → 8)Kdo(2 → 4)Kdo-BSA, the inset shows linear fitting (lines) and the initial data points (open black circles). ( D ) Standard curves for S67-26 Fab concentration assay based on the steady state of initial mass transport limited rates from the sensorgrams in ( C ). The fitting to a solution affinity model of data for the inhibition of ( E ) S67-27 Fab binding to 570 RUs of immobilized Kdo(2 → 8)Kdo(2 → 4)Kdo-BSA by various saccharides. Kdo(2 → 4)Kdo; Kdo(2 → 8)-7- O -Me-Kdo; Kdo(2 → 8)Kdo; Kdo; Kdo(2 → 8)Kdo(2 → 4)Kdo; Kdo(2 → 4)Kdo(2 → 4)Kdo; Ko.

Brooks, C. L., Blackler, R. J., Sixta, G., Kosma, P., Müller-Loennies, S., Brade, L., ... & Evans, S. V. (2009). The role of CDR H3 in antibody recognition of a synthetic analog of a lipopolysaccharide antigen. Glycobiology, 20(2), 138-147.