Mouse Anti-BCHE Recombinant Antibody (clone 11D8) (CAT#: FAMAB-0028CQ)

Figure 1 Reactivity of anti-HuBChE monoclonal antibodies with BChE in the plasma or sera from various animal species.

A) measured with Pansorbin cells; B) measured with Dynabeads protein G.

Peng, H., Brimijoin, S., Hrabovska, A., Krejci, E., Blake, T. A., Johnson, R. C., ... & Lockridge, O. (2016). Monoclonal antibodies to human butyrylcholinesterase reactive with butyrylcholinesterase in animal plasma. Chemico-biological interactions, 243, 82-90.

Figure 2 Human BChE is recognized by monoclonal antibody 11D8

Selectivity of 11D8 for recombinant and wild-type BChE by ELISA (60 min detection)

Hrabovska, A., Bernard, V., & Krejci, E. (2010). A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains. PLoS One, 5(9), e12892.

Figure 3 Human BChE is recognized by monoclonal antibody 11D8

Immunohistochemistry of human neuromuscular junction (NMJ). Top, nicotinic acetylcholine receptors are visualized with bungarotoxin (Bung). Bottom, staining with 11D8 labels BChE.

Hrabovska, A., Bernard, V., & Krejci, E. (2010). A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains. PLoS One, 5(9), e12892.

Figure 4 Human BChE is recognized by monoclonal antibody 11D8

11D8 shifts BChE in sucrose gradient. Sucrose gradient of human PRiMA-BChE complex extracted from COS cells. Binding of 11D8 shifts the peaks of tetramer and dimmer. Wider high-S peak suggests a mixed population of IgG/one tetramer and IgG/two tetramers.

Hrabovska, A., Bernard, V., & Krejci, E. (2010). A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains. PLoS One, 5(9), e12892.

Figure 5 Human BChE is recognized by monoclonal antibody 11D8

11D8 recognizes BChE expressed by COS cells, anchored by ColQ and PRiMA as documented by confocal microscopy

Hrabovska, A., Bernard, V., & Krejci, E. (2010). A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains. PLoS One, 5(9), e12892.

Figure 6 Antibodies from BChE knockout mice immunized with mouse BChE.

Western blot of recombinant mouse BChE with sera from mice immunized with recombinant mouse BChE (m1–m3), or from mice immunized with BChE purified from human serum (h1–h6), or with monoclonal antibody produced against human low-glycosylated BChE (11D8). In this experiment, each antibody sample was tested with 75 ng, 38 ng and 8 ng of recombinant mouse BChE loaded onto a denaturing gel. In the 11D8 lane, the band marked with "*" represents 750 ng of protein.

Hrabovska, A., Bernard, V., & Krejci, E. (2010). A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains. PLoS One, 5(9), e12892.