Human Anti-HIV-1 Env Recombinant Antibody (clone CH239) (CAT#: MRO-2779CQ)
Recombinant human antibody to Env. CH239 can neutralize HIV-1 isolates.
Specific Inquiry
Neu
Figure 1 Neutralization Activity of the CH235 Clonal Lineage Antibodies against Autologous CH505 Viruses.
Heatmap analysis of neutralization data generated from 41 pseudoviruses (row) and 10 CH235 lineage mAbs (column). The neutralization potency (IC50) is shown in different shades of colors as indicated in the histogram, from white (>50 μg/ml) to dark red (0.1 μg/ml).
Gao, F., Bonsignori, M., Liao, H. X., Kumar, A., Xia, S. M., Lu, X., ... & Lynch, R. M. (2014). Cooperation of B cell lineages in induction of HIV-1-broadly neutralizing antibodies. Cell, 158(3), 481-491.
Neu
Figure 2 Neutralization Activity of antibodies against CH505 Env Loop D Mutants.
Heat map analysis of neutralization data generated from ten loop D mutants (row) and ten CH235 lineage mAbs (column). The neutralization potency (IC50) is shown in different shades of colors as indicated in the histogram, from white (>50 μg/ml) to dark red (0.1 μg/ml).
Gao, F., Bonsignori, M., Liao, H. X., Kumar, A., Xia, S. M., Lu, X., ... & Lynch, R. M. (2014). Cooperation of B cell lineages in induction of HIV-1-broadly neutralizing antibodies. Cell, 158(3), 481-491.
SPR
Figure 3 Binding Kinetics of Lineage Antibodies.
Binding association (ka) and dissociation (kd) rates of the lineage mAbs to CH505. TF gp120 Env were measured with SPR and used to calculate the dissociation rate constants (Kd).
Bonsignori, M., Zhou, T., Sheng, Z., Chen, L., Gao, F., Joyce, M. G., ... & Alam, S. M. (2016). Maturation pathway from germline to broad HIV-1 neutralizer of a CD4-mimic antibody. Cell, 165(2), 449-463.
ELISA
Figure 4 CH235 Antibody Lineage Autoreactivity and Polyreactivity.
Binding to cardiolipin was determined using Quanta Lite ACA IgG III ELISA assay.
Bonsignori, M., Zhou, T., Sheng, Z., Chen, L., Gao, F., Joyce, M. G., ... & Alam, S. M. (2016). Maturation pathway from germline to broad HIV-1 neutralizer of a CD4-mimic antibody. Cell, 165(2), 449-463.
FC
Figure 5 CH235 Antibody Lineage Autoreactivity and Polyreactivity.
Measurement of polyreactivity against 9,400 human antigens using ProtoArray 5 microchip: CH235 lineage mAbs binding (x axis) was compared to nonpolyreactive control mAb 151K (y axis). Polyreactivity is defined as 1 log stronger binding than 151k mAb to more than 90% of the test proteins. High affinity binding was measured as a>2 log increase in binding (dotted line).
Bonsignori, M., Zhou, T., Sheng, Z., Chen, L., Gao, F., Joyce, M. G., ... & Alam, S. M. (2016). Maturation pathway from germline to broad HIV-1 neutralizer of a CD4-mimic antibody. Cell, 165(2), 449-463.
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Recognizes HIV-1 Env
- Species Reactivity
- HIV-1
- Clone
- CH239
- Applications
- Neut, SPR, ELISA, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Env antibody has been reported in applications of Neutralization, Surface Plasmon Resonance, Enzyme-linked Immunosorbent Assay, Flow Cytometry.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160; precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone CH239"
See other products for "HIV-1 Env"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-057 | Recombinant Anti-HIV-1 env VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-474-F(E) | Recombinant Human Anti-HIV-1 Env Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| PFBL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124); Fab Fragment | WB, Neut, FuncS | Human Fab |
| PFBL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22); Fab Fragment | WB, ELISA, Neut, FuncS | Human Fab |
| PFBL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L); Fab Fragment | WB, ELISA, IF, FuncS | Human Fab |
| PFBL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11); Fab Fragment | WB, ELISA, FuncS | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124) | WB, Neut, FuncS | Human IgG |
| PABL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L) | WB, ELISA, IF, FuncS | Human IgG |
| PABL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11) | WB, ELISA, FuncS | Human IgG |
| PABL-145 | Human Anti-HIV-1 Env Recombinant Antibody (PABL-145) | ELISA, Neut, FuncS | Human IgG1, κ |
MHC Tetramer for Virology
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-420 | Human Anti-HIV-1 Env Recombinant Antibody (clone CAP228-3D); scFv Fragment | Neut | Human scFv |
| HPAB-2324LY-S(P) | Mouse Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (HPAB-2324LY-S(P)) | Neut, ELISA | Mouse scFv |
| HPAB-2325LY-S(P) | Mouse Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (HPAB-2325LY-S(P)) | Neut, ELISA | Mouse scFv |
| PABJ-0039-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone 1-55); scFv Fragment | ELISA, Neut | Human scFv |
| PABJ-0053-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone 1-18); scFv Fragment | ELISA, Neut | Human scFv |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for MRO-2779CQ. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.