Recombinant Human Anti-PSIP1 Single Domain Antibody (PABC-550) (CAT#: PABC-550)

Figure 1 Human Anti-PSIP1 Single Domain Antibody (VH59) in Neut.

VH59 disrupts the interaction of LEGDF IBD with HIV IN in Jurkat T cell inhibiting the production of viral capsid protein p24.

Bao, L. , Hannon, C. , Cruz-Mignoni, A. , Ptchelkine, D. , Sun, M. Y. , & Miller, A. , et al. Intracellular immunization against hiv infection with an intracellular antibody that mimics hiv integrase binding to the cellular ledgf protein. Scientific Reports.

Figure 2 Human Anti-PSIP1 Single Domain Antibody (VH59) in Luciferase.

Interaction of LEGDF IBD and anti-IBD VH59 in mammalian cells. The interaction of the LEGDF IBD with the iDab VH59 was tested by mammalian two-hybrid analysis using a luciferase reporter system in CHO cells. LEDGF IBD or LMO2 control baits were expressed as fusions with GAL4 DBD (pM1 vector) and various VH iDAbs or HIV IN were expressed as prey fusions with the VP16 activation domain (pEF-VP16 vector). Luciferase reporter activity in transfected CHO cells. Forty-eight hours after transfection, cells were lysed and luciferase activities were measured. Firefly/Renilla luciferase activity was calculated for each transformation pair.

Bao, L. , Hannon, C. , Cruz-Mignoni, A. , Ptchelkine, D. , Sun, M. Y. , & Miller, A. , et al. Intracellular immunization against hiv infection with an intracellular antibody that mimics hiv integrase binding to the cellular ledgf protein. Scientific Reports.