Rabbit Anti-PSIP1 Recombinant Antibody (clone R52-3N-5)
CAT#: VS3-XY3478
This is a recombinant rabbit antibody targeting the PSIP1 (PC4 and SRSF1 interacting protein 1), which may play an important role in lens epithelial to fiber cell terminal differentiation. PSIP1 may also play a protective role during stress-induced apoptosis. It specifically reacts with human PSIP1. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear as well as cytoplasmic positivity in neuronal and glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/178_B4_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/247_B4_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/177_B4_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46099_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining:High
Intensity: Strong
Endothelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Enterocytes
Staining:High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:High
Intensity: Strong
Goblet cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_7_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HEL)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164985-PSIP1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PSIP1
- Species Reactivity
- Human
- Clone
- R52-3N-5
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Interstitial Cystitis; Laryngeal Small Cell Carcinoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/100-1/200
IF: 1/50-1/200
Target
- Alternative Names
- p52; p75; PAIP; DFS70; LEDGF; PSIP2
- Gene ID
- 11168
- UniProt ID
- O75475
- Long Name
- PC4 and SRSF1 interacting protein 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Citrullinated by PADI4. (O75475-PSIP1_HUMAN)
Ubiquitination at Lys6, Lys75, Lys364, Lys407, Lys415, Lys422, Lys424, and Lys442 (NX_O75475 [NX_O75475-1])
Modification sites at PhosphoSitePlus (O75475)
Glycosylation from GlyGen (O75475) 2 sites, 2 O-linked glycans (2 sites)
- Protein Refseq
- XP_024303168.1
- Function
- Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis.
Involved in particular in lens epithelial cell gene regulation and stress responses.
May play an important role in lens epithelial to fiber cell terminal differentiation.
May play a protective role during stress-induced apoptosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PSIP1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-487 | Recombinant Human Anti-Human PSIP1 Antibody, clone D484 | ELISA, IP-MS | IgG |
| MOB-1701CT | Recombinant Mouse anti-Human PSIP1 Monoclonal antibody (7F5) | ICC, IF, IHC-P, WB | |
| VS3-XY1312 | Mouse Anti-PSIP1 Recombinant Antibody (clone 6E4) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| VS7-0425-WR702 | Mouse Anti-PSIP1 Monoclonal Antibody (VS7-0425-WR702) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2886 | Hi-Affi™ Recombinant Rabbit Anti-PSIP1 Monoclonal Antibody (DS2886AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-550 | Recombinant Human Anti-PSIP1 Single Domain Antibody (PABC-550) | ELISA, WB, Inhib | Human VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY415 | Mouse Anti-NHP PSIP1 Recombinant Antibody (clone 6E4) | WB, IHC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1773 | Anti-PSIP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5771 | Anti-Mouse PSIP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5770 | Anti-Human PSIP1 Immunohistochemistry Kit | IHC |
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