Anti-Human GDF15 Recombinant Antibody (A10.3) (CAT#: TAB-0782CL)
Recombinant monoclonal antibody A10.3 is a mouse monoclonal antibody that specifically binds to human macrophage inhibitory factor 1 and can be potentially used in the treatment of pancreatic cancer.
Specific Inquiry
IHC
Figure 1 IHC for GDF15 on two human ovarian tumor tissue arrays.
A tumor tissue array consisting of 29 tumor tissue samples and 5 non-cancerous ovarian tissue samples was stained for GDF15.
Griner, S. E., Joshi, J. P., & Nahta, R. (2013). Growth differentiation factor 15 stimulates rapamycin-sensitive ovarian cancer cell growth and invasion. Biochemical pharmacology, 85(1), 46-58.
ELISA
Figure 2 GDF15 promotes anchorage-independent growth of ovarian cancer cells.
CaOv3, OvCar3, and SKOv3 cells were incubated in serum-free media for 48 h. GDF15 concentration was then determined by ELISA in media from cell lines.
Griner, S. E., Joshi, J. P., & Nahta, R. (2013). Growth differentiation factor 15 stimulates rapamycin-sensitive ovarian cancer cell growth and invasion. Biochemical pharmacology, 85(1), 46-58.
IHC
Figure 3 Rapamycin inhibits GDF15-mediated ovarian cancer cell invasion.
SKOv3 cells were plated in Boyden invasion chambers in the presence of vehicle control, 20 ng/ mL GDF15, or GDF15 plus 100 nM rapamycin.
Griner, S. E., Joshi, J. P., & Nahta, R. (2013). Growth differentiation factor 15 stimulates rapamycin-sensitive ovarian cancer cell growth and invasion. Biochemical pharmacology, 85(1), 46-58.
WB
Figure 4 GDF15 knockdown reduces invasion and growth in association with reduced p-4EBP1 in GDF15-overexpressing ovarian cancer cells.
Western blots were performed at least twice for phosphorylated and total 4EBP1, Erk1/2, p38, and Akt; representative blots are shown.
Griner, S. E., Joshi, J. P., & Nahta, R. (2013). Growth differentiation factor 15 stimulates rapamycin-sensitive ovarian cancer cell growth and invasion. Biochemical pharmacology, 85(1), 46-58.
Specifications
- Immunogen
- PCan065 Construct
- Host Species
- Mouse
- Specificity
- Human
- Clone
- A10.3
- Applications
- ELISA, IHC, WB
- Related Disease
- Breast cancer, colon cancer, lung cancer and pancreatic cancer
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The GDF15 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry. ELISA: The sample media was incubated in GDF15 antibody-coated microplates for 2 h, then
washed and incubated with GDF15 antibody conjugated to
horseradish peroxidase for1 h.After washing, plates were incubated
with color reagent(hydrogen peroxide–chromogen mix)for 30 min.
Target
- Alternative Names
- GDF15; growth differentiation factor 15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; growth/differentiation factor 15; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; prostate differentiation factor; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1
- Gene ID
- 9518
- UniProt ID
- Q99988
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-391-S(P) | Recombinant Anti-human GDF15 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| HPAB-M0525-YC-S(P) | Human Anti-GDF15 Recombinant Antibody; scFv Fragment (HPAB-M0525-YC-S(P)) | ELISA, Block | Human scFv |
| HPAB-M0526-YC-S(P) | Human Anti-GDF15 Recombinant Antibody; scFv Fragment (HPAB-M0526-YC-S(P)) | ELISA, Block | Human scFv |
| HPAB-M0527-YC-S(P) | Human Anti-GDF15 Recombinant Antibody; scFv Fragment (HPAB-M0527-YC-S(P)) | ELISA, Block | Human scFv |
| HPAB-M0528-YC-S(P) | Human Anti-GDF15 Recombinant Antibody; scFv Fragment (HPAB-M0528-YC-S(P)) | ELISA, Block | Human scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-391 | Recombinant Human Anti-human GDF15 Antibody | ELISA, WB, FuncS | IgG |
| MOB-0141CT | Recombinant Mouse anti-Human GDF15 Monoclonal antibody (NN0408-20B4) | FC, WB | |
| HPAB-0334-CN | Mouse Anti-GDF15 Recombinant Antibody (HPAB-0334-CN) | ELISA, WB | Mouse IgG1, κ |
| HPAB-M0527-YC | Human Anti-GDF15 Recombinant Antibody (HPAB-M0527-YC) | ELISA, Block | Human IgG |
| HPAB-M0528-YC | Human Anti-GDF15 Recombinant Antibody (HPAB-M0528-YC) | ELISA, Block | Human IgG |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0781CL | Anti-Human GDF15 Recombinant Antibody | ELISA, WB | Humanized antibody |
| TAB-0781CL-S(P) | Anti-Human GDF15 Recombinant Antibody scFv Fragment | ELISA, WB | Humanized antibody |
| TAB-0781CL-F(E) | Anti-Human GDF15 Recombinant Antibody Fab Fragment | ELISA, WB | Humanized antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0783CL | Anti-Human GDF15 Recombinant Antibody (B2.2) | ELISA, FC | |
| TAB-0785CL | Mouse Anti-GDF15 Recombinant Antibody (TAB-0785CL) | ELISA, FC, WB | Mouse IgG |
| TAB-0786CL | Mouse Anti-GDF15 Recombinant Antibody (TAB-0786CL) | ELISA, FC, WB | Mouse IgG |
| TAB-0787CL | Mouse Anti-GDF15 Recombinant Antibody (TAB-0787CL) | ELISA, FC, WB | Mouse IgG |
| TAB-0788CL | Mouse Anti-GDF15 Recombinant Antibody (TAB-0788CL) | ELISA, FC, WB | Mouse IgG |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0784CL | Human Anti-GDF15 Recombinant Antibody (TAB-0784CL) | ELISA, FC | Human IgG |
| TAB-0784CL-S(P) | Human Anti-GDF15 Recombinant Antibody; scFv Fragment (TAB-0784CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0784CL-F(E) | Human Anti-GDF15 Recombinant Antibody; Fab Fragment (TAB-0784CL-F(E)) | ELISA, FC | Human Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1421 | Hi-Affi™ Rabbit Anti-GDF15 Recombinant Antibody (clone DS1421AB) | WB | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0334-CN-F(E) | Mouse Anti-GDF15 Recombinant Antibody; Fab Fragment (HPAB-0334-CN-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-0335-CN-F(E) | Mouse Anti-GDF15 Recombinant Antibody; Fab Fragment (HPAB-0335-CN-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-0336-CN-F(E) | Mouse Anti-GDF15 Recombinant Antibody; Fab Fragment (HPAB-0336-CN-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-0337-CN-F(E) | Human Anti-GDF15 Recombinant Antibody; Fab Fragment (HPAB-0337-CN-F(E)) | ELISA, WB | Human Fab |
| HPAB-0142-YJ-F(E) | Human Anti-GDF15 Recombinant Antibody; Fab Fragment (HPAB-0142-YJ-F(E)) | Inhib, FuncS | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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