Staphylococcus aureus V-8 Protease also known as endoproteinase Glu-C, has two pH optima, one at pH 4.0 and the other at pH 7.8, and is specific for cleavage at the carboxy terminus of glutamic acid and aspartic acid residues. The protease is specific only for glutamic digestion in buffers which do not contain phosphate at either pH optimum. Phosphate buffers at pH 7.8 will facilitate digestion at both glutamic and aspartic acid residues.
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