Recombinant Anti-GFAP Single Domain Antibody (HPAB-1158-FY) (CAT#: HPAB-1158-FY)
This product is a recombinant Single Domain Antibody that recognizes GFAP. The monoclonal Single Domain Antibody E3 reacts with the epitope of GFAP.
Specific Inquiry
WB
Figure 1 Western blot analyses of anti-GFAP specificities.
GFAP and human brain extracts were electrophoresed, immunoblotted and incubated with the different VHHs as described in ''Materials and methods''.
Perruchini, C., Pecorari, F., Bourgeois, J. P., Duyckaerts, C., Rougeon, F., & Lafaye, P. (2009). Llama V H H antibody fragments against GFAP: better diffusion in fixed tissues than classical monoclonal antibodies. Acta neuropathologica, 118(5), 685.
Specifications
- Host Species
- Llama
- Type
- Llama VHH
- Specificity
- Human GFAP
- Species Reactivity
- Human
- Clone
- HPAB-1158-FY
- Applications
- WB, ELISA, IHC
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The GFAP antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
WB: following the separation by polyacrylamide gel electrophoresis (PAGE) using NuPAGE Novex 4–12% Bis–Tris gel, semi-dry transfer onto Hybond-C and Western blotting were carried out using the Xcell II blot module. Before the immunochemical reaction, membranes were blocked in a 4% skimmed milk solution. Immunoblotting of membranes was accomplished with VHH (VHH A10 1 μg/ml, VHH E9 and VHH E3 0.1 μg/ml), and revealed by anti-His-tag 1:1,000 followed by peroxidaselabeled goat anti-rabbit immunoglobulins 1:10,000. Finally, peroxidase activity was visualized using a chemiluminescent kit.
Target
- Alternative Names
- Glial Fibrillary Acidic Protein; Intermediate Filament Protein; ALXDRD
- Gene ID
- 2670
- UniProt ID
- P14136
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GFAP"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-068 | Recombinant Anti-Mouse Gfap VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-083CQ | Llama Anti-GFAP Recombinant Antibody (TAB-083CQ) | IHC, WB, IF | Llama VHH |
| TAB-084CQ | Llama Anti-GFAP Recombinant Antibody (TAB-084CQ) | WB | Llama VHH |
| HPAB-1159-FY | Recombinant Anti-GFAP Single Domain Antibody (HPAB-1159-FY) | WB, ELISA, IHC | Llama VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2053MZ | Recombinant Mouse Anti-Human GFAP Antibody (clone 7B7) | ELISA, ICC/IF, IHC-P, WB | Mouse antibody |
| HPAB-0197-WJ | Mouse Anti-GFAP Recombinant Antibody (clone 5C8) | ELISA, IHC | Mouse IgG1 |
| ZG-0074C | Mouse Anti-GFAP Recombinant Antibody (clone ABT-GFAP) | IHC-P, IF | Mouse IgG |
| ZG-0075C | Mouse Anti-GFAP Recombinant Antibody (clone PT1995) | IHC-P, IF | Mouse IgG |
| ZG-0385J | Mouse Anti-GFAP Recombinant Antibody (ZG-0385J) | WB, IHC, IF, ELISA | Mouse IgG |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0700MZ | Chicken Anti-GFAP Polyclonal IgY | ICC/IF, IHC, WB | Chicken antibody |
| BRD-0805MZ | Chicken Anti-GFAP Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1122MZ | Chicken Anti-GFAP Polyclonal IgY | IHC, IF, WB | Chicken antibody |
| BRD-1174MZ | Chicken Anti-Glial Fibrillary Acidic Protein (GFAP) Polyclonal IgY | WB, ICC | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1426 | Hi-Affi™ Rabbit Anti-GFAP Recombinant Antibody (clone DS1426AB) | ICC, IHC-Fr, IHC-P, IP, WB | Rabbit IgG |
| MOR-0100-FY | Rabbit Anti-GFAP Recombinant Antibody (clone AFY0071) | ICC, IF, IHC-P, WB | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0197-WJ-S(P) | Mouse Anti-GFAP Recombinant Antibody (clone 5C8); scFv Fragment | ELISA, IHC | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0197-WJ-F(E) | Mouse Anti-GFAP Recombinant Antibody (clone 5C8); Fab Fragment | ELISA, IHC | Mouse Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-1158-FY. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.