Mouse Anti-GFAP Recombinant Antibody (clone ABT-GFAP)
CAT#: ZG-0074C
This product is a mouse antibody that recognizes GFAP. It can be used in a wide variety of applications, such as: IHC-P, IF.
Tested Data
Gene Expression
IHC
Figure 2 Mouse Anti-GFAP Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Brain. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 3 Mouse Anti-GFAP Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Brain. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 4 Mouse Anti-GFAP Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Brain. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 5 Mouse Anti-GFAP Antibody in IHC
Immunohistochemical analysis of paraffin-embedded Glioma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
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Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
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Specifications
- Immunogen
- Synthesized peptide derived from human Glial Fibrillary Acidic Protein
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Glial Fibrillary Acidic
- Species Reactivity
- Human, Mouse
- Clone
- ABT-GFAP
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence. For details, refer to Tested Data.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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|---|---|---|---|
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