Mouse Anti-GFAP Recombinant Antibody (ZG-0385J)
CAT#: ZG-0385J
This product is a mouse antibody that recognizes GFAP.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-GFAP Recombinant Antibody (ZG-0385J) in WB
Western Blot analysis using GFAP Monoclonal Antibody against A431 (1), SK-N-SH (2) and PC12 (3) cell lysate.
IHC
Figure 2 Mouse Anti-GFAP Recombinant Antibody (ZG-0385J) in IHC
Immunohistochemistry analysis of paraffin-embedded brain tissues with DAB staining using GFAP Monoclonal Antibody.
IF
Figure 3 Mouse Anti-GFAP Recombinant Antibody (ZG-0385J) in IF
Immunofluorescence analysis of paraffin-embedded lobe of brain tissues using GFAP Monoclonal Antibody (green).
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Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
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Specifications
- Immunogen
- Purified recombinant fragment of human GFAP expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GFAP
- Species Reactivity
- Human
- Applications
- WB, IHC, IF, ELISA
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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