What makes this antibody "ADCC Enhanced"?

This Anti-ICAM1 Antibody (BI-505) is produced using our Afuco™ platform, which utilizes a fucosyltransferase-engineered cell line. This results in an afucosylated antibody that exhibits enhanced Antibody-Dependent Cellular Cytotoxicity (ADCC) compared to standard IgG1 antibodies.

Afuco™ Anti-Human ICAM1 ADCC Recombinant Antibody (BI-505), ADCC Enhanced

CAT#: AFC-361CL

Anti-ICAM1 ADCC Enhanced Antibody (BI-505) is an ADCC enhanced antibody produced by our Afuco™ platform. BI-505 is a fully human high-affinity IgG1 antibody, which was isolated from a highly diverse human phage-antibody library based on its functional ability to induce programmed cell death of tumor cells. BI-505 binds to intercellular adhesion molecule-1 (ICAM-1), suggesting a previously unrecognized role for this receptor as a therapeutic target in cancer.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/29_G3_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2142/203_G3_1_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line EFO-21

Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/1937_A10_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line GAMG

Immunofluorescent staining of human cell line GAMG shows localization to plasma membrane, vesicles, nucleoplasm & centrosome.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/2058_A10_4_blue_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_B_9_5.jpg

Normal Tissue Figure 6 Lung

Alveolar cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_A_3_4.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_3.jpg

Normal Tissue Figure 8 Kidney

Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_5.jpg

Normal Tissue Figure 9 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_9_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (HHSteC, Karpas-707, RPMI-8226)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000090339-ICAM1

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Specifications

Host Species
Human

Derivation
Human

Type
ADCC enhanced antibody

Antibody Isotype
IgG1

Species Reactivity
Human

Related Disease
Multiple Myeloma

Product Property

Purity
>95% as judged by SDS-polyacrylamide gel electrophoresis

Storage
≥1 year at -20°C. If the reconstituted antibody cannot be used within two weeks, it should be aliquoted into smaller vials and stored at -20°C

Target

Alternative Names
ICAM1; intercellular adhesion molecule 1; BB2; CD54; P3.58; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor

Gene ID
3383

UniProt ID
P05362

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Breast cancer biomarkers at key points during disease progression

Enhanced ADCC Activity

We were interested in the ADCC capabilities of this antibody, and the Afuco™ platform really delivers. Clone BI-505 showed enhanced ADCC activity in our assays compared to standard antibodies. It binds high-affinity to ICAM1 and induces cell death effectively in our tumor models.

High Affinity Human Antibody

This fully human IgG1 antibody is of excellent quality. We isolated it for its functional ability to bind ICAM-1, and it has proven to be a robust reagent. The purity is>95% by SDS-PAGE, and it has been stable when stored at -20°C.

Q&As

What makes this antibody "ADCC Enhanced"?

A: This Anti-ICAM1 Antibody (BI-505) is produced using our Afuco™ platform, which utilizes a fucosyltransferase-engineered cell line. This results in an afucosylated antibody that exhibits enhanced Antibody-Dependent Cellular Cytotoxicity (ADCC) compared to standard IgG1 antibodies.
Is this a fully human antibody?

A: Yes, BI-505 is a fully human high-affinity IgG1 antibody. It was isolated from a highly diverse human phage-antibody library based on its functional ability to induce programmed cell death of tumor cells by binding to ICAM-1.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Rheumatoid Arthritis

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Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AFC-361CL.pdf

MSDS

MSDS - AFC-361CL.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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