anti-ICAM1 immunotoxin UV3 (IgG)-RTA (CAT#: AGTO-L023R)
This immunotoxin was achieved by conjugating/fusing the Anti-ICAM1 monoclonal antibody to ricin toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to melanoma cells. Then the ricin A chain is incorporated into the cell by receptor-mediated endocytosis, blocking ribosomal protein synthesis and ultimately leading to cell death. It was designed for treatment of melanoma.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/29_G3_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/29_G3_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2142/203_G3_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2142/203_G3_1_selected.jpg
Figure 3 IF staining of human cell line EFO-21
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/1937_A10_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/1937_A10_1_blue_red_green.jpg
Figure 4 IF staining of human cell line GAMG
(Immunofluorescent staining of human cell line GAMG shows localization to plasma membrane, vesicles, nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/2058_A10_4_blue_red_green.jpg
(Immunofluorescent staining of human cell line GAMG shows localization to plasma membrane, vesicles, nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/2058_A10_4_blue_red_green.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_B_9_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_B_9_5.jpg
Figure 6 Lung
(Alveolar cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_A_3_4.jpg
(Alveolar cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_A_3_4.jpg
Figure 7 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_3.jpg
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_3.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_5.jpg
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_5.jpg
Figure 9 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_9_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_9_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (HHSteC, Karpas-707, RPMI-8226)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000090339-ICAM1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000090339-ICAM1
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- UV3
- Molecule Class
- L kappa; IgG2a-RTA
- Toxin Description
- Ricin consist of two polypeptide chains, A and B, linked by means of disulfide bridge(s). Cytotoxicity is associated with the A chain and its 15 inhibition of protein synthesis in nucleated cells. The B chain is essentially a delivery vehicle for the A chain. The B chain recognizes polysaccharide units at the surface of cells and creates a high affinity interaction with such units. Once the B chain binds with polysaccharide units at 20" the celL surface, the A chain is incorporated into the cell, blocking ribosomal protein synthesis and ultimately leading tocell death. The use of ricin A chain is preferred in this invention.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Melanoma
Target
- Alternative Names
- ICAM1; intercellular adhesion molecule 1; BB2; CD54; P3.58; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Melanoma; MM; Deglycosylated ricin A chain; Ricin A chain; Ricin A; Ricin; RTA; RT; RA; dgRTA; dgRA; dgA
- Gene ID
- 3383
- UniProt ID
- P05362
Related Resources
Rheumatoid Arthritis
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TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1937-VHH | Recombinant Anti-human ICAM1 VHH Single Domain Antibody | FA, FC, ICC, ELISA | Llama VHH |
| TAB-126CT | Llama Anti-ICAM1 Recombinant Antibody (TAB-126CT) | ELISA, IF | Llama VHH |
| HPAB-0349-YC-VHH | Recombinant Llama Anti-ICAM1 Single Domain Antibody (34-1) | ELISA | Llama VHH |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-241 | Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (Enlimomab Pegol) | WB, IF, IP, Neut, FuncS, ELISA, FC | Fab' - G2a |
| TAB-H26 | Anti-Human ICAM1 Recombinant Antibody (Enlimomab) | Neut, ELISA, IF, IP, FuncS, FC, WB | IgG2a |
| TAB-124CT | Anti-Human ICAM-1 Recombinant Antibody (m1A6) | ELISA | |
| TAB-124CT-S(P) | Anti-Human ICAM-1 Recombinant Antibody scFv Fragment (m1A6) | ELISA | |
| TAB-124CT-F(E) | Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (m1A6) | ELISA |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-376CL | Human Anti-ICAM1 Recombinant Antibody (TAB-376CL) | IHC, FuncS | Human IgG1 |
| TAB-121CT | Anti-Human ICAM-1 Recombinant Antibody (MD-2) | ELISA, FC | Human antibody |
| TAB-122CT | Anti-Human ICAM-1 Recombinant Antibody (R6-5-D6) | ELISA, FC | Human antibody |
| TAB-125CT-S(P) | Anti-Human ICAM-1 Recombinant Antibody scFv Fragment (ICM 10064) | Inhibit, ELISA, WB | Human antibody |
| TAB-125CT-F(E) | Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (ICM 10064) | Inhibit, ELISA, WB | Human antibody |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-079 | Mouse Anti-ICAM1 Recombinant Antibody (clone CA7); Fab Fragment | FC | Mouse Fab |
| HPAB-0043-FY-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone Icmo0191); Fab Fragment | ELISA | Human Fab, λ |
| HPAB-0859-CN-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone BI-AB); Fab Fragment | ELISA, FC, Neut | Human Fab |
| HPAB-662-FY-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone ICM10088); Fab Fragment | Inhib | Human Fab |
| HPAB-1728-FY-S(P) | Human Anti-ICAM1 Recombinant Antibody (clone LAC1555); Fab Fragment | ELISA | Human Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-123CT | Anti-Human ICAM-1 Recombinant Antibody (h1A6) | ELISA | Humanized antibody |
| TAB-123CT-S(P) | Anti-Human ICAM-1 Recombinant Antibody scFv Fragment (h1A6) | ELISA | Humanized antibody |
| TAB-123CT-F(E) | Anti-Human ICAM-1 Recombinant Antibody Fab Fragment (h1A6) | ELISA | Humanized antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-104LC | Recombinant Anti-Human ICAM1 Antibody (Fc glycosylation) | ELISA | Mouse antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-923CQ | Mouse Anti-ICAM1 Recombinant Antibody (clone CBL236) | Neut, IF, FC, WB, IHC | Mouse IgG1 |
| NEUT-925CQ | Mouse Anti-ICAM1 Recombinant Antibody (clone HA58) | FC, FuncS, IHC, Neut, WB | Mouse IgG1, κ |
| NEUT-926CQ | Mouse Anti-ICAM1 Recombinant Antibody (VMC182) | FC, IF, IHC, ICC, Neut | Mouse IgG2b |
| NEUT-927CQ | Mouse Anti-ICAM1 Recombinant Antibody (clone RR1/1) | FC, IF, IHC, Neut, WB, ELISA | Mouse IgG1 |
| NEUT-933CQ | Mouse Anti-Icam1 Recombinant Antibody (clone 1A29) | WB, IP, ICC, IF, IHC-P, Neut, FC | Mouse IgG1 |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-924CQ | Mouse Anti-ICAM1 Recombinant Antibody (clone HCD54) | FC, IF, Block | Mouse IgG1, κ |
| NEUT-928CQ | Mouse Anti-ICAM1 Recombinant Antibody (VMC183) | Block, FC, IHC, IP | Mouse IgG1 |
| NEUT-929CQ | Mouse Anti-ICAM1 Recombinant Antibody (clone BBIG-I1(11C81)) | Block, WB, IP, ICC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1723 | Rabbit Anti-ICAM1 Recombinant Antibody (clone DS1723AB), Biotin | IHC-Fr, IP, WB, ELISA | Rabbit IgG |
| MOR-1724 | Rabbit Anti-Icam1 Recombinant Antibody (clone DS1724AB) | ELISA | Rabbit IgG |
| MOR-4598 | Rabbit Anti-ICAM1 Recombinant Antibody (clone TH111DS) | WB, FC | Rabbit IgG |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-1635LC | Mouse Anti-ICAM1 Recombinant Antibody (clone c78.4A) | FC | Mouse IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0859-CN-S(P) | Human Anti-ICAM1 Recombinant Antibody (clone BI-AB); scFv Fragment | ELISA, FC, Neut | Human scFv |
| HPAB-1728-FY-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone LAC1555); scFv Fragment | ELISA | Human scFv |
| HPAB-1729-FY-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone LAC3040); scFv Fragment | ELISA | Human scFv |
| HPAB-1730-FY-F(E) | Human Anti-ICAM1 Recombinant Antibody (clone LAC3041); scFv Fragment | ELISA | Human scFv |
| HPAB-S0065-YC-S(P) | Human Anti-ICAM1 Recombinant Antibody (clone HumF); scFv Fragment | ELISA, FuncS | Humanized scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0062-YC | Human Anti-ICAM1 Recombinant Antibody (clone HumB) | ELISA, FuncS | Humanized IgG |
| HPAB-S0063-YC | Human Anti-ICAM1 Recombinant Antibody (clone HumC) | ELISA, FuncS | Humanized IgG |
| HPAB-S0064-YC | Human Anti-ICAM1 Recombinant Antibody (clone HumD) | ELISA, FuncS | Humanized IgG |
| HPAB-S0065-YC | Human Anti-ICAM1 Recombinant Antibody (clone HumF) | ELISA, FuncS | Humanized IgG |
| HPAB-S0066-YC | Human Anti-ICAM1 Recombinant Antibody (clone HumH) | ELISA, FuncS | Humanized IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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