Recombinant Mouse Anti-Mouse Prnp Antibody (POM11) (CAT#: EPAF-0834CQ)

This product is a mouse antibody that specifically recognizes Prnp from mouse. The antibody POM11 is an epitope-specific antibody that can be used in Inhib and other immunological assays.


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SDS-PAGE

Figure 1 Mouse Anti-Mouse Prnp Antibody (EPAF-0834CQ) in SDS-PAGE

Figure 1 Mouse Anti-Mouse Prnp Antibody (EPAF-0834CQ) in SDS-PAGE

SDS-PAGE analysis of EPAF-0834CQ in reduced (Lane 2) and non-reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

HPLC

Figure 2 Mouse Anti-Mouse Prnp Antibody (EPAF-0834CQ) in HPLC

Figure 2 Mouse Anti-Mouse Prnp Antibody (EPAF-0834CQ) in HPLC

The purity of EPAF-0834CQ was greater than 95% as determined by SEC-HPLC.

WB

Figure 3 Mouse Anti-Mouse Prnp Antibody (EPAF-0834CQ) in WB

Figure 3 Mouse Anti-Mouse Prnp Antibody (EPAF-0834CQ) in WB

WB analysis of EPAF-0834CQ was performed by loading with a polypeptide-BSA from Mouse Prnp (aa 65-89).

The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)

Lane M: Marker
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

ELISA

Figure 4 Mouse Anti-Mouse Prnp Antibody (EPAF-0834CQ) in ELISA

Figure 4 Mouse Anti-Mouse Prnp Antibody (EPAF-0834CQ) in ELISA

ELISA analysis of EPAF-0834CQ was performed by coating with a polypeptide-BSA from Mouse Prnp (aa 65-89).

The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)

DB

Figure 5 Mouse Anti-Mouse Prnp Antibody (EPAF-0834CQ) in DB

Figure 5 Mouse Anti-Mouse Prnp Antibody (EPAF-0834CQ) in DB

Dot Blot analysis of EPAF-0834CQ was performed by coating with a polypeptide-BSA from Mouse Prnp (aa 65-89).

EPAF-0834CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)


Specifications

  • Host Species
  • Mouse
  • Type
  • IgG1
  • Specificity
  • Mouse Major prion protein
  • Species Reactivity
  • Mouse
  • Clone
  • POM11
  • Applications
  • Used for immunoassay techniques such as: Inhibition
  • Conjugate
  • Unconjugated

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Storage
  • Aliquot and store at -20°C long term. Avoid repeated freeze-thaw cycles.

Target

  • Alternative Names
  • Prnp; Major prion protein; Mouse Prnp; Mouse

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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