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Rabbit Anti-SMAD3 Polyclonal Antibody (CAT#: VS3-WK1563)

This antibody is a rabbit polyclonal antibody against human SMAD3, which functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. It was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen (synthesized peptide derived from human SMAD3). It can detect endogenous levels of SMAD3. This product was tested for use in WB and ELISA.


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Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46386/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human parathyroid gland
Figure 3 IHC staining of human parathyroid gland
(Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/ihc_selected.jpg
Figure 4 IHC staining of human duodenum
Figure 4 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_7_2_selected.jpg
Figure 5 Lymph node
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_8_8.jpg
Figure 6 Testis
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166949-SMAD3

Specifications

  • Immunogen
  • Synthesized peptide derived from human Smad3
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human SMAD3
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • WB, ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Polyclonal
  • Purification
  • Affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • 1 mg/mL
  • Buffer
  • PBS, 0.5% BSA, 50% Glycerol
  • Preservative
  • 0.02% Sodium Azide
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot (1:500) and ELISA (1:10000-1/20000).

Target

  • Alternative Names
  • SMAD Family Member 3; Mothers Against DPP Homolog 3; MAD Homolog 3; JV15-2; HMAD-3; HSMAD3; MADH3; MAD3; MAD, Mothers Against Decapentaplegic Homolog 3 (Drosophila); SMAD, Mothers Against DPP Homolog 3 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 3; Mothers Against Decapentaplegic Homolog 3
  • Long Name
  • SMAD Family Member 3
  • Sequence Similarities
  • Belongs to the dwarfin/SMAD family.
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Phosphorylated on serine and threonine residues. Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF and TGF-beta treatment. Ser-208 is the main site of MAPK-mediated phosphorylation. CDK-mediated phosphorylation occurs in a cell-cycle dependent manner and inhibits both the transcriptional activity and antiproliferative functions of SMAD3. This phosphorylation is inhibited by flavopiridol.
  • Function
  • Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes.

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Related Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "SMAD3"

Recombinant Antibody

CAT Product Name Application Type
MOB-3147z Mouse Anti-SMAD3 Recombinant Antibody (clone 24F4) WB, ELISA, IHC Mouse IgG1, κ
MOB-1886CT Recombinant Mouse anti-Human SMAD3 Monoclonal antibody (EML539) IHC-P, WB
MRO-2380-CN Recombinant Rabbit Anti-SMAD3 (phosphorylated Ser423/S425) Monoclonal Antibody (CBACN-665) WB, IF, IHC Rabbit IgG
ZG-094R Mouse Anti-Smad3 Recombinant Antibody (ZG-094R) WB, ELISA, IF Mouse IgG
VS3-FY1358 Recombinant Rabbit Anti-SMAD3 Antibody (clone R02-1I2) WB, IP Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3322 Hi-Affi™ Recombinant Rabbit Anti-SMAD3 Monoclonal Antibody (DS3322AB) WB, ICC, IHC-P, IP, sELISA IgG
MOR-0052-FY Rabbit Anti-SMAD3 Recombinant Antibody (clone AFY0023) ICC, IHC-P, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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