Recombinant Rabbit Anti-SMAD3 Antibody (clone R02-1I2) (CAT#: VS3-FY1358)

This product is a recombinant rabbit antibody that recognizes SMAD3. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R02-1I2 is specific for human, mouse, rat, Hamster SMAD3. The antigen is human smad3 recombinant protein.


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WB

Figure 1. Recombinant Rabbit Anti-SMAD3 Antibody (clone R02-1I2) in WB.

Figure 1. Recombinant Rabbit Anti-SMAD3 Antibody (clone R02-1I2) in WB.

Western Blot analysis of Smad3 in 3T3, C6 lysates using Smad3 Antibody.

WB

Figure 2. Recombinant Rabbit Anti-SMAD3 Antibody (clone R02-1I2) in WB.

Figure 2. Recombinant Rabbit Anti-SMAD3 Antibody (clone R02-1I2) in WB.

Western Blot analysis of Smad3 in Jurkat lysates using Smad3 antibody.


Specifications

  • Immunogen
  • Human Smad3 recombinant protein.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat, Hamster SMAD3
  • Species Reactivity
  • Human, Mouse, Rat, Hamster
  • Clone
  • R02-1I2
  • Applications
  • Western Blot, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 48 kDa; Observed MW: 52 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • SMAD Family Member 3; Mothers Against DPP Homolog 3; MAD Homolog 3; JV15-2; HMAD-3; HSMAD3; MADH3; MAD3; MAD, Mothers Against Decapentaplegic Homolog 3 (Drosophila); SMAD, Mothers Against DPP Homolog 3 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 3; Mothers Against Decapentaplegic Homolog 3

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Related Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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