Monkey Anti-HIV-1 gp120 Recombinant Antibody (clone 2.2G) (CAT#: MRO-3536CQ)

Figure 1 HIV-1 SF162 neutralization susceptibility by RhMAbs.

The neutralizing activity of the RhMAbs against HIV-1 SF162 was determined using the TZM-bl pseudovirus neutralization assay.

Robinson, J. E., Franco, K., Elliott, D., Maher, M. J., Reyna, A. A., Montefiori, D. C., ... & Stamatatos, L. (2010). Quaternary Epitope Specificities of Anti-HIV-1 Neutralizing Antibodies Generated in Rhesus Macaques Infected by the Simian/Human Immunodeficiency Virus SHIVSF162P4. Journal of Virology, 84(7), 3443-3453.

Figure 2 Competing the capturing of virions by RhMAbs with human MAbs.

SF162 virions were first incubated with the human anti-HIV-1 Env MAbs 447 (anti-V3) or soluble CD4 and then added to wells coated with the indicated MAbs (x axis). Inhibition of capturing is represented as the percent reduction in virion capturing (y axis) compared to the capturing of SF162 virions that were not preincubated with human MAbs or sCD4.

Robinson, J. E., Franco, K., Elliott, D., Maher, M. J., Reyna, A. A., Montefiori, D. C., ... & Stamatatos, L. (2010). Quaternary Epitope Specificities of Anti-HIV-1 Neutralizing Antibodies Generated in Rhesus Macaques Infected by the Simian/Human Immunodeficiency Virus SHIVSF162P4. Journal of Virology, 84(7), 3443-3453.

Figure 3 Effect of V2 loop NLGSs on the neutralizing activity of RhMAb 2.2G.

The V2 glycosylation sites (154, 186, and 195) were eliminated from the SF162 Env by mutating either the asparagines to glutamines or the serines to alanines.

Robinson, J. E., Franco, K., Elliott, D., Maher, M. J., Reyna, A. A., Montefiori, D. C., ... & Stamatatos, L. (2010). Quaternary Epitope Specificities of Anti-HIV-1 Neutralizing Antibodies Generated in Rhesus Macaques Infected by the Simian/Human Immunodeficiency Virus SHIVSF162P4. Journal of Virology, 84(7), 3443-3453.

Figure 4 Effect of variable loop deletion on virion capture by human and rhesus MAbs.

SF162-derived virions without or with deletions in the V1, V2, or V3 loop (DV1, DV2, or DV3, respectively) were added to ELISA wells coated with the indicated MAbs. MAbs 2909, 3.9F, and IgGb12 are human MAbs directed to quaternary epitope, V3, and CD4-binding domain, respectively.

Robinson, J. E., Franco, K., Elliott, D., Maher, M. J., Reyna, A. A., Montefiori, D. C., ... & Stamatatos, L. (2010). Quaternary Epitope Specificities of Anti-HIV-1 Neutralizing Antibodies Generated in Rhesus Macaques Infected by the Simian/Human Immunodeficiency Virus SHIVSF162P4. Journal of Virology, 84(7), 3443-3453.