Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d)

(A) HIV-1 isolates 89.6, ADA, and SG3 were preincubated for 1 h with a fixed total concentration (0.3 μg/ml for 89.6 and ADA and 0.005 μg/ml for SG3) but various ratios of VRC01-E51 mixtures. TZM-bl cells were then added and incubated for 48 h. Infection was measured by the percent luciferase expression in the absence of inhibitor. (B) Experiment similar to that for panel A except that the indicated isolates were preincubated for 1 h with a fixed total concentration (0.3 μg/ml for 89.6 and ADA and 1.0 μg/ml for SA32) but various ratios of VRC01-412d mixtures.

Gardner, M. R., Fellinger, C. H., Prasad, N. R., Zhou, A. S., Kondur, H. R., Joshi, V. R.,... & Farzan, M. (2016). CD4-induced antibodies promote association of the HIV-1 envelope glycoprotein with CD4-binding site antibodies. Journal of virology, JVI-00803.

(A) HIV-1 isolates BG505 and ConC were preincubated for 1 h with various concentrations of VRC01, E51, 412d, and 17b. TZM-bl cells were then added and incubated for 48 h. Infection was measured by the percent luciferase expression in the absence of inhibitor. (B) Similar to panel A except that BG505 or ConC was preincubated for 1 h with a fixed total concentration (0.033 μg/ml for BG505 or 0.045 μg/ml for ConC) but various ratios of VRC01-E51 mixtures. (C) Similar to panel A except that BG505 or ConC was preincubated for 1 h with a fixed total concentration (0.033 μg/ml for BG505 or 0.045 μg/ml for ConC) but various ratios of VRC01-412d mixtures.

Gardner, M. R., Fellinger, C. H., Prasad, N. R., Zhou, A. S., Kondur, H. R., Joshi, V. R.,... & Farzan, M. (2016). CD4-induced antibodies promote association of the HIV-1 envelope glycoprotein with CD4-binding site antibodies. Journal of virology, JVI-00803.

The experiment was similar to that described for Fig. 1 except that HIV-1 isolates 89.6, ADA, SG3, and SA32 was preincubated with various concentrations of indicated antibodies or mixture of antibodies. The dashed lines indicate the fixed concentration used for Fig. 1A and B. For the 89.6 and ADA neutralization curves, 50:50 mixtures of VRC01 and E51, VRC01 and 412d, b12 and E51, and VRC01 and 2G12 were used. For the SG3 neutralization curves, 25:75 mixtures of VRC01 and E51, b12 and E51, and VRC01 and 2G12 were used, while a 75:25 mixture of VRC01 and 412d was used (to accommodate for the lack of potency of 412d on SG3). For the SA32 neutralization curves, a 40:60 ratio of VRC01 and E51 was used, while 60:40 ratios of VRC01 and 412d, b12 and 412d, and VRC01 and 2G12 were used.

Gardner, M. R., Fellinger, C. H., Prasad, N. R., Zhou, A. S., Kondur, H. R., Joshi, V. R.,... & Farzan, M. (2016). CD4-induced antibodies promote association of the HIV-1 envelope glycoprotein with CD4-binding site antibodies. Journal of virology, JVI-00803.

(A) The cells were preincubated with E51 at serially diluted concentrations starting at 50 μg/ml. Cells were washed and then incubated with 0.01 μg/ml of the indicated CD4bs antibody or CD4-Ig. Analysis was performed as for Fig. 4 except that MFI data were normalized to the value of each antibody or CD4-Ig binding without E51 preincubation. (B) Experiment similar to that for panel A except that cells were preincubated with 50 μg/ml of 412d, 17b, or CCR5mim2-Ig. Note that the scale of the y axes varies between graphs.

Gardner, M. R., Fellinger, C. H., Prasad, N. R., Zhou, A. S., Kondur, H. R., Joshi, V. R.,... & Farzan, M. (2016). CD4-induced antibodies promote association of the HIV-1 envelope glycoprotein with CD4-binding site antibodies. Journal of virology, JVI-00803.

Xiang, S. H., Farzan, M., Si, Z., Madani, N., Wang, L., Rosenberg, E.,... & Sodroski, J. (2005). Functional mimicry of a human immunodeficiency virus type 1 coreceptor by a neutralizing monoclonal antibody. Journal of virology, 79(10), 6068-6077.

Xiang, S. H., Farzan, M., Si, Z., Madani, N., Wang, L., Rosenberg, E.,... & Sodroski, J. (2005). Functional mimicry of a human immunodeficiency virus type 1 coreceptor by a neutralizing monoclonal antibody. Journal of virology, 79(10), 6068-6077.

Veillette, M., Désormeaux, A., Medjahed, H., Gharsallah, N. E., Coutu, M., Baalwa, J.,... & Haynes, B. F. (2014). Interaction with cellular CD4 exposes HIV-1 envelope epitopes targeted by antibody-dependent cell-mediated cytotoxicity.Journal of virology, 88(5), 2633-2644.