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Rabbit Anti-EEF2 Polyclonal Antibody (CAT#: VS3-WK1560)

This antibody is a rabbit polyclonal antibody against human EEF2, which promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. It was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. It can detect endogenous levels of eEF2. This product was tested for use in WB and ELISA.


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Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane, cytosol & aggresome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7795/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_B_8_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_8_3.jpg
Figure 5 Testis
Figure 5 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_6_6.jpg
Figure 6 Ovary
Figure 6 Ovary
(Follicle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Ovarian stroma cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_4_7.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167658-EEF2

Specifications

  • Immunogen
  • Synthesized peptide derived from human eEF2
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human EEF2
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • WB, ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Polyclonal
  • Purification
  • Affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • 1 mg/mL
  • Buffer
  • PBS, 0.5% BSA, 50% Glycerol
  • Preservative
  • 0.02% Sodium Azide
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot (1:500) and ELISA (1:10000-1/20000).

Target

  • Alternative Names
  • Eukaryotic Translation Elongation Factor 2; Polypeptidyl-TRNA Translocase; EF-2; EF2; Elongation Factor 2; EEF-2; SCA26
  • Long Name
  • Eukaryotic Translation Elongation Factor 2
  • Sequence Similarities
  • Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine (By similarity).
    (Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
  • Function
  • Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "EEF2"

Immunotoxin

CAT Product Name Application Type
AGTO-G017E Anti-EEF2 immunotoxin G4S (scFv)-PE Cytotoxicity assay, Function study
AGTO-G017D Anti-EEF2 immunotoxin G4S (scFv)-DT Cytotoxicity assay, Function study

Recombinant Antibody

CAT Product Name Application Type
MOB-2361MZ Recombinant Mouse Anti-Human EEF2 Antibody (clone FMM3B22I3) WB Mouse antibody
ZG-0271J Mouse Anti-EEF2 Recombinant Antibody (ZG-0271J) WB, ICC Mouse IgG
ZG-0272J Mouse Anti-EEF2 Recombinant Antibody (ZG-0272J) WB Mouse IgG
ZG-0273J Mouse Anti-EEF2 Recombinant Antibody (ZG-0273J) WB, IHC, IF, ELISA Mouse IgG
VS3-QX337 Mouse Anti-EEF2 Recombinant Antibody (clone 4B3-G7-H5) WB, ICC Mouse IgG2b

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1096 Hi-Affi™ Rabbit Anti-EEF2 Recombinant Antibody (clone DS1096AB) WB, IHC-P, ICC, IF Rabbit IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC1929 PE-A*02:01/Human EEF2 (ILTDITKGV) MHC Tetramer FCM
MHC-LC4409 PE-A*01:01/Human EEF2 (YFDPANGKF) MHC Tetramer FCM

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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