Hi-Affi™ Rabbit Anti-EEF2 Recombinant Antibody (clone DS1096AB)
CAT#: MOR-1096
Recombinant Rabbit anti-EEF2 monoclonal antibody for WB, IHC-P, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane, cytosol & aggresome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7795/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_8_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_6_6.jpg
Normal Tissue
Figure 6 Ovary
Follicle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Ovarian stroma cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_4_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167658-EEF2
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Specifications
- Immunogen
- A synthetic peptide corresponding to residues near the N-terminus of human eEF2 was used as immunogen.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human EEF2
- Species Reactivity
- Human
- Clone
- DS1096AB
- Applications
- WB, IHC-P, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Tissue culture supernatant
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "EEF2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2641z | Mouse Anti-EEF2 Recombinant Antibody (clone 26G3) | WB, ELISA, ICC, IF, IHC | Mouse IgG1 |
| MOB-2361MZ | Recombinant Mouse Anti-Human EEF2 Antibody (clone FMM3B22I3) | WB | Mouse antibody |
| VS3-WK160 | Mouse Anti-EEF2 Recombinant Antibody (clone H1-B6) | WB, ELISA | Mouse IgG1 |
| VS3-FY1999 | Rabbit Anti-EEF2 Recombinant Antibody (clone R07-2C0) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
| VS7-HM838 | Mouse Anti-EEF2 Recombinant Antibody (clone 5B6) | WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G017E | Anti-EEF2 immunotoxin G4S (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G017D | Anti-EEF2 immunotoxin G4S (scFv)-DT | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1929 | PE-A*02:01/Human EEF2 (ILTDITKGV) MHC Tetramer | FCM | |
| MHC-LC4409 | PE-A*01:01/Human EEF2 (YFDPANGKF) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY733 | Anti-EEF2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2168 | Anti-Mouse EEF2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2167 | Anti-Human EEF2 Immunohistochemistry Kit | IHC |
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