Anti-EEF2 immunotoxin G4S (scFv)-PE
CAT#: AGTO-G017E
This immunotoxin was achieved by conjugating/fusing the Anti-EEF2 scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to leukemia cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating T cell leukemia.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane, cytosol & aggresome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7795/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_8_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_6_6.jpg
Normal Tissue
Figure 6 Ovary
Follicle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Ovarian stroma cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_4_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40534/138197_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167658-EEF2
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Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- G4S
- Molecule Class
- scFv-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- T cell leukemia
Target
- Alternative Names
- EEF2; eukaryotic translation elongation factor 2; EF2; EF-2; EEF-2; SCA26; elongation factor 2; polypeptidyl-tRNA translocase; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE43
- Gene ID
- 1938
- UniProt ID
- P13639
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High Cytotoxicity In Leukemia Cells
We utilized this immunotoxin for our cytotoxicity assays involving T cell leukemia lines. The product demonstrated efficient receptor-mediated endocytosis and subsequent cleavage of the PE toxin, leading to significant inhibition of protein synthesis as expected in our experimental design.
Effective Protein Synthesis Inhibition
The mechanism of action involving the ADP ribosylation of elongation factor 2 was clearly observed in our functional studies. The fusion of the Anti-EEF2 scFv to the Pseudomonas exotoxin provided a highly specific tool for our research into apoptosis induction pathways.
Q&As
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What is the mechanism of action for this immunotoxin?
A: This product functions by binding to the target cells via receptor-mediated endocytosis. Once inside, the Pseudomonas exotoxin (PE) is cleaved, and the fragment translocates to the Golgi, where it inactivates elongation factor 2 (EEF2) via ADP ribosylation, inhibiting protein synthesis and causing apoptosis.
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How is this immunotoxin expressed and purified?
A: We produce this immunotoxin by expressing it in CHO (Chinese Hamster Ovary) cells. It is subsequently purified using affinity chromatography to ensure high quality and specificity for use in cytotoxicity and functional assays targeting T cell leukemia.
View the frequently asked questions answered by Creative Biolabs Support.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone G4S"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2641z | Mouse Anti-EEF2 Recombinant Antibody (clone 26G3) | WB, ELISA, ICC, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| AGTO-G017D | Anti-EEF2 immunotoxin G4S (scFv)-DT | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MHC-LC1929 | PE-A*02:01/Human EEF2 (ILTDITKGV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
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| ZG-0271J | Mouse Anti-EEF2 Recombinant Antibody (ZG-0271J) | WB, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
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| ZG-0272J | Mouse Anti-EEF2 Recombinant Antibody (ZG-0272J) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
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| ZG-0273J | Mouse Anti-EEF2 Recombinant Antibody (ZG-0273J) | WB, IHC, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
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| VS-0325-XY733 | Anti-EEF2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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