Mouse Anti-Type 1 poliovirus Capsid protein VP1 Recombinant Antibody (Clone C3) (CAT#: PABS-0045)

Figure 1 Reactivity of the peptide conjugate with monoclonal antibodies.

The KLH-peptide conjugate or KLH alone were labeled with ¹²⁵I using chloramine T and immunoprecipitated as indicated. Panel A: 200 ul (1.56 x 10⁶ c.p.m.) of peptide-coupled KLH at 5 ug/ml (8.5 ug/ml of KLH) were immunoprecipitated using increasing amounts of the neutralizing monoclonal antibody C3 (open circles), or of the non-neutralizing monoclonal antibody, C18 (closed circles). Panel B: 200 ul (1.56 x 10⁶c.p.m.) of KLH at 8.5 ug/ml (closed circles) or of peptide conjugate (open circles) were immunoprecipitated using increasing amounts of the neutralizing monoclonal antibody C3. Immune precipitates were collected on protein A-Sepharose and assayed for radioactivity. Background values (1.76 x 10⁴ c.p.m.) obtained with protein A-Sepharose alone were subtracted.

Wychowski, C., Van der Werf, S., Siffert, O., Crainic, R., Bruneau, P., & Girard, M. (1983). A poliovirus type 1 neutralization epitope is located within amino acid residues 93 to 104 of viral capsid polypeptide VP1. The EMBO Journal, 2(11), 2019-2024.

Figure 2 Neutralization inhibition test.

The amount of monoclonal antibody(a 4^-6 dilution of C3 ascites fluid) just sufficient to neutralize 150 p.f.u./ml of poliovirus was mixed in a total volume of 0.25 ml with the indicated amounts of peptide. After 2 h incubation at 37°C, each tube received 0.25 ml of virus, and incubation was continued for an additional 2 h. The infectious virus remaining in each sample was titrated by plaque assay, and the titer compared with that found after incubation in the absence of monoclonal antibody (166 p.f.u./ml) or in the absence of peptide (25 p.f.u./ml).

Wychowski, C., Van der Werf, S., Siffert, O., Crainic, R., Bruneau, P., & Girard, M. (1983). A poliovirus type 1 neutralization epitope is located within amino acid residues 93 to 104 of viral capsid polypeptide VP1. The EMBO Journal, 2(11), 2019-2024.