Recombinant monoclonal antibody to CXCR5. Clone 40C01-VH1-Vk3 is a human monoclonal antibody that can be potentially used in treatment or prevention of CXCR5 related diseases or disorders.
Figure 1 Inhibition of CXCL13-stimulated chemotaxis of L1.2 cells expressing-human (panel A to F) and cynomolgus monkey (G and H) CXCR5, caused by anti-CXCR5 40C01-VH1-Vk3 and 40C01-VH2-Vk3 variants expressed in a whole antibody format as IgG1, IgG2 and IgG4.
The results were normalized for basal activity (medium alone) and the maximal response obtained with 10 nM CXCL13 (100%). The displayed data are representative of three independent experiments. All points were run in triplicate (error bars indicate ±S.E.M).
Figure 2 Inhibition of human CXCL13-stimulated chemotaxis of primary human B cells caused by anti-CXCR5 40C01-VH1-Vk3 (panel A) and 40C01-VH2-Vk3 (panel B) variants expressed in a whole antibody format as IgG1.
The results are expressed as calculated IC50s (half maximal inhibitory concentration). Values are the means±S.E.M from 8 (40C01-VH1-Vk3) and 5 (40C01-VH2-Vk3) separate experiments respectively.
Figure 3 Binding of Alexa-Fluor 647 labelled anti-CXCR5 40C01 variants to HEK-293 cells stably transfected with human CXCR5.
Figure 4 Binding of Alexa-Fluor 647-labelled anti-CXCR5 40C01 variants to human B cells in whole blood.
Figure 5 Binding of anti-CXCR5 40C01 variants to cynomolgus monkey B cells in whole blood.
Figure 6 Inhibition of CXCL13-stimulated ERK phosphorylation in B cells in human whole blood by anti-CXCR5 mAbs.
Figure 7 Inhibition of CXCL13-stimulated ERK phosphorylation in B cells in cynomolgus monkey whole blood by anti-CXCR5 mAbs.
Figure 8 Determination of the KD of anti-CXCR5 mAb 40C01-VH1-Vk3 variant for cell-membrane expressed CXCR5 using KinExA methodology.
CXCR5-HEK-293 cells (5×10⁶/ml) were serially diluted and incubated with 30 pM (solid diamonds) or 300 pM (open circles) active binding site concentration of anti-CXCR5 mAb 40C01-VH1-Vk3, in the presence of 0.02% NaN3 and allowed to equilibrate. The free mAb left in the supernatant was measured. (A) The % free mAb is plotted against the antigen concentration (arbitrarily taking each million cells to be equal to 10−9 M antigen). Multiple curve analysis ("n-curve analysis") using the unknown antigen method was performed to determine optimal values for KD and the antigen multiplier (B and C respectively) The 95% confidence intervals were determined by iteratively changing the optimized value for KD or antigen multiplier while keeping other parameters at their optimal values.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-156MZ | Human Anti-CXCR5 Recombinant Antibody (TAB-156MZ) | FC | Human IgG1 |
TAB-157MZ | Human Anti-CXCR5 Recombinant Antibody (TAB-157MZ) | FC | Human IgG1 |
TAB-158MZ | Human Anti-CXCR5 Recombinant Antibody (TAB-158MZ) | FC | Human IgG1 |
TAB-159MZ | Human Anti-CXCR5 Recombinant Antibody (TAB-159MZ) | FC | Human IgG1 |
TAB-160MZ | Human Anti-CXCR5 Recombinant Antibody (TAB-160MZ) | FC | Human IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-161MZ | Anti-Human CXCR5 Recombinant Antibody (m2C9) | ELISA, FC, FuncS, Inhib | |
TAB-162MZ | Anti-Human CXCR5 Recombinant Antibody (m2B5) | ELISA, FC | |
TAB-165MZ | Mouse Anti-CXCR5 Recombinant Antibody (TAB-165MZ) | ELISA | Mouse IgG4 |
TAB-161MZ-F(E) | Anti-Human CXCR5 Recombinant Antibody Fab Fragment (m2C9) | ELISA, FC | |
TAB-162MZ-F(E) | Anti-Human CXCR5 Recombinant Antibody Fab Fragment (m2B5) | ELISA, FC |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-163MZ | Anti-Human CXCR5 Recombinant Antibody (h2C9) | ELISA, FC, FuncS, Inhib | Humanized antibody |
TAB-164MZ | Anti-Human CXCR5 Recombinant Antibody (h2B5) | ELISA, FC | Humanized antibody |
TAB-166MZ | Human Anti-CXCR5 Recombinant Antibody (TAB-166MZ) | ELISA | Humanized IgG |
TAB-163MZ-F(E) | Anti-Human CXCR5 Recombinant Antibody Fab Fragment (h2C9) | ELISA, FC | Humanized antibody |
TAB-164MZ-F(E) | Anti-Human CXCR5 Recombinant Antibody Fab Fragment (h2B5) | ELISA, FC | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-128LC | Recombinant Anti-Human CXCR5 Antibody (Fc glycosylation/Low fucosylated) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-128LC-1 | Recombinant Anti-Human CXCR5 Antibody (Fc glycosylation/Low fucosylated) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2070MZ | Recombinant Mouse Anti-Human CXCR5 Antibody (clone 62606.222) | FC | Mouse antibody |
HPAB-0110-LSX | Human Anti-CXCR5 Recombinant Antibody (clone H4BL2) | ELISA, WB, FC, Dep | Human IgG |
HPAB-0111-LSX | Mouse Anti-CXCR5 Recombinant Antibody (HPAB-0111-LSX) | RIA, FC, WB | Mouse IgG |
HPAB-0112-LSX | Human Anti-CXCR5 Recombinant Antibody (HPAB-0112-LSX) | RIA, FC, WB | Human IgG |
HPAB-0113-LSX | Human Anti-CXCR5 Recombinant Antibody (HPAB-0113-LSX) | RIA, FC, WB | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-695CQ | Mouse Anti-CXCR5 Recombinant Antibody (clone 8C17) | FC, IHC, Neut | Mouse IgG2 |
NEUT-696CQ | Mouse Anti-CXCR5 Recombinant Antibody (clone CBL047) | FC, WB, IHC | Mouse IgG2 |
NEUT-697CQ | Mouse Anti-CXCR5 Recombinant Antibody (clone CBL475) | FC, IHC, CyTOF®, ICC, Neut | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0890 | Hi-Affi™ Rabbit Anti-CXCR5 Recombinant Antibody (clone DS890AB) | WB, ICC, FC | Rabbit IgG |
MOR-4565 | Hi-Affi™ Rabbit Anti-CXCR5 Recombinant Antibody (clone TH75DS) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0109-LSX-S(P) | Mouse Anti-CXCR5 Recombinant Antibody (clone 2C9); scFv Fragment | ELISA, WB | Mouse scFv |
HPAB-0110-LSX-S(P) | Human Anti-CXCR5 Recombinant Antibody (clone H4BL2); scFv Fragment | ELISA, WB | Humanized scFv |
HPAB-0111-LSX-S(P) | Mouse Anti-CXCR5 Recombinant Antibody; scFv Fragment (HPAB-0111-LSX-S(P)) | RIA, FC, WB | Mouse scFv |
HPAB-0112-LSX-S(P) | Mouse Anti-CXCR5 Recombinant Antibody; scFv Fragment (HPAB-0112-LSX-S(P)) | RIA, FC, WB | Mouse scFv |
HPAB-0113-LSX-S(P) | Human Anti-CXCR5 Recombinant Antibody; scFv Fragment (HPAB-0113-LSX-S(P)) | RIA, FC, WB | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0109-LSX-F(E) | Mouse Anti-CXCR5 Recombinant Antibody (clone 2C9); Fab Fragment | ELISA, WB | Mouse Fab |
HPAB-0110-LSX-F(E) | Human Anti-CXCR5 Recombinant Antibody (clone H4BL2); Fab Fragment | ELISA, WB | Humanized Fab |
HPAB-0111-LSX-F(E) | Mouse Anti-CXCR5 Recombinant Antibody; Fab Fragment (HPAB-0111-LSX-F(E)) | RIA, FC, WB | Mouse Fab |
HPAB-0112-LSX-F(E) | Human Anti-CXCR5 Recombinant Antibody; Fab Fragment (HPAB-0112-LSX-F(E)) | RIA, FC, WB | Chimeric (mouse/human) Fab |
HPAB-0113-LSX-F(E) | Human Anti-CXCR5 Recombinant Antibody; Fab Fragment (HPAB-0113-LSX-F(E)) | RIA, FC, WB | Humanized Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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