Human Anti-Adenylate cyclase toxin Recombinant Antibody (clone 3D1) (CAT#: FAMAB-1305CQ)
This product is a recombinant human antibody that recognizes Adenylate cyclase toxin. The antibody was purified by affinity chromatography. It can be used in IP, Neut, WB, ELISA, FC.
Specific Inquiry
WB
Figure 1 CyaA entrains CD11b/CD18 into lipid rafts.
Unless otherwise stated, CyaA was detected using the 9D4 monoclonal antibody (MAb) recognizing the C-terminal RTX repeats. The 3D1 MAb was used to specifically detect the distal part (aa 373-400) of the AC domain of CyaA.
Bumba, L., Masin, J., Fiser, R., & Sebo, P. (2010). Bordetella adenylate cyclase toxin mobilizes its β 2 integrin receptor into lipid rafts to accomplish translocation across target cell membrane in two steps. PLoS Pathog, 6(5), e1000901.
IF
Figure 2 Mobilization of CyaA into lipid rafts depends on CyaA-mediated Ca2+ influx.
J774A.1 cells were incubated at 37uC for 10 min with 6 nM CyaA proteins labeled with Alexa Fluor 488 before 5 mg/ml of Alexa Fluor 594-labeled recombinant cholera toxin subunit B (CtxB) was added for additional 5 min.
Bumba, L., Masin, J., Fiser, R., & Sebo, P. (2010). Bordetella adenylate cyclase toxin mobilizes its β 2 integrin receptor into lipid rafts to accomplish translocation across target cell membrane in two steps. PLoS Pathog, 6(5), e1000901.
Specifications
- Immunogen
- Bordetella pertussis adenylate cyclase toxin
- Host Species
- Human
- Type
- Human IgG1
- Species Reactivity
- B. pertussis
- Clone
- 3D1
- Applications
- WB, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot and Immunofluorescence.
Target
- Alternative Names
- Adenylate cyclase toxin
- UniProt ID
- C8C508
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1306CQ | Human Anti-Adenylate cyclase toxin Recombinant Antibody (clone 9D4) | WB | Human IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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