This product is antibody recognizes EGFRvIII. The antibody D2C7 can be used in some immunoassay techniques such as: FC, In vitro cytotoxicity assay.
Figure 1 Determination of the epitope of D2C7 on EGFRvIII ECD.
The wells were blocked with superblock and incubated with D2C7 mAb followed by peroxidase conjugated anti-mouse IgG antibody. Following the addition of TMB substrate solution, and stop solution, the optical density was measured at 450 nm. The ELISA assay with different concentrations of Nus-Tag alone or Nus-Tag conjugated to EGFRvIII ECD deletion mutants was conducted in triplicate for each epitope.
Chandramohan, V., Bao, X., Keir, S. T., Pegram, C. N., Szafranski, S. E., Piao, H., ... & Bigner, D. D. (2013). Construction of an immunotoxin, D2C7-(scdsFv)-PE38KDEL, targeting EGFRwt and EGFRvIII for brain tumor therapy. Clinical Cancer Research, 19(17), 4717-4727.
Figure 2 Flow cytometric analysis of D2C7-(scdsFv)-PE38KDEL to determine reactivity of the D2C7 immunotoxin.
Parental NR6 cells were used as control. Indirect FACS analysis shows the reactivity of D2C7- (scdsFv)-PE38KDEL immunotoxin with cells expressing EGFRwt (NR6W).
Chandramohan, V., Bao, X., Keir, S. T., Pegram, C. N., Szafranski, S. E., Piao, H., ... & Bigner, D. D. (2013). Construction of an immunotoxin, D2C7-(scdsFv)-PE38KDEL, targeting EGFRwt and EGFRvIII for brain tumor therapy. Clinical Cancer Research, 19(17), 4717-4727.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-310 | Recombinant Anti-EGFRvIII Antibody | ELISA, WB, Dot, FuncS | IgG |
HPAB-648-FY | Human Anti-EGFRvIII Recombinant Antibody (HPAB-648-FY) | FC, ELISA | Human IgG |
HPAB-0138-YJ | Human Anti-EGFRvIII Recombinant Antibody (HPAB-0138-YJ) | ELISA, Inhib, FuncS | Human IgG1, κ |
HPAB-M0065-YC | Human Anti-EGFRvIII Recombinant Antibody (HPAB-M0065-YC) | FC, Block, FuncS | Human IgG |
HPAB-0662-WJ | Human Anti-EGFRvIII Recombinant Antibody (HPAB-0662-WJ) | ELISA | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-310-F(E) | Recombinant Anti-EGFRvIII Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
HPAB-M0065-YC-F(E) | Human Anti-EGFRvIII Recombinant Antibody; Fab Fragment (HPAB-M0065-YC-F(E)) | FC, Block, FuncS | Human Fab |
HPAB-M0066-YC-F(E) | Mouse Anti-EGFRvIII Recombinant Antibody; Fab Fragment (HPAB-M0066-YC-F(E)) | FC | Mouse Fab |
HPAB-M0067-YC-F(E) | Human Anti-EGFRvIII Recombinant Antibody; Fab Fragment (HPAB-M0067-YC-F(E)) | FC | Human Fab |
HPAB-M0270-YC-F(E) | Human Anti-EGFRvIII Recombinant Antibody; Fab Fragment (HPAB-M0270-YC-F(E)) | FC | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-310-S(P) | Recombinant Human Anti-EGFRvIII Antibody scFv Fragment | IF, Neut, FuncS | scFv |
HPAB-0483LY-S(P) | Human Anti-EGFRvIII Recombinant Antibody (clone CBL009LY); scFv Fragment | ELISA | Human scFv |
HPAB-648-FY-S(P) | Human Anti-EGFRvIII Recombinant Antibody; scFv Fragment (HPAB-648-FY-S(P)) | FC, ELISA | Human scFv |
HPAB-M0270-YC-S(P) | Human Anti-EGFRvIII Recombinant Antibody; scFv Fragment (HPAB-M0270-YC-S(P)) | FC | Human scFv |
HPAB-0662-WJ-S(P) | Human Anti-EGFRvIII Recombinant Antibody; scFv Fragment (HPAB-0662-WJ-S(P)) | ELISA | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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