Recombinant Mouse Anti-PRV gB Antibody (1H1) (CAT#: PABC-493)

Figure 1 Neutralizing efficacies of 1H1 mAb against different PRV strains.

Purified 1H1 mAb was used for virus neutralizing assay to test its efficacy against 8 different PRV strains, including vaccine strains (Bartha and HB98), classical virulent strains (RA and SU), and the current emerging variant strains (HN1201, 188–5, 072–1, and BH1). The neutralizing profile of each strain is represented by different symbols as indicated in the legend.

Li, X., Yang, F., Hu, X., Tan, F., Qi, J., Peng, R., ... & Bai, C. (2017). Two classes of protective antibodies against Pseudorabies virus variant glycoprotein B: Implications for vaccine design. PLoS pathogens, 13(12), e1006777.

Figure 2 Flow cytometry analysis to verify the 1H1 epitope by mutagenesis.

The 293T cells were transfected by wild type (A) PRV gB or mutants (B-F) expression vectors to allow protein expression on the cell surface. The 1H1 mAb was then used to stain the transfected cells, which were further stained by APC.linked secondary antibody. The fluorescence signals of the cells were visualized and quantified by flow cytometry. The profiles of cells transfected with pEGFP-N1 empty vectors are represented by solid black areas (negative control) and the cells transfected with gB or mutant expression plasmids are represented by red silhouettes.

Li, X., Yang, F., Hu, X., Tan, F., Qi, J., Peng, R., ... & Bai, C. (2017). Two classes of protective antibodies against Pseudorabies virus variant glycoprotein B: Implications for vaccine design. PLoS pathogens, 13(12), e1006777.