Mouse Anti-STEAP1 Recombinant Antibody (clone 120.545) (CAT#: PABS-0208)

Figure 1 Cell-surface ferric-reductase activity of STEAP4 and STEAP4/1 chimera while varying the concentration of added Fab120.545.

The STEAP4/1 chimera datapoints are fitted with a one-phase decay curve.

Oosterheert, W., & Gros, P. (2020). Structural basis for targeting human cancer antigen STEAP1 with antibodies. bioRxiv.

Figure 2 Cell-surface ferric-reductase activity of STEAP protein variants supplemented with PBS or with 4 µM Fab120.545.

Oosterheert, W., & Gros, P. (2020). Structural basis for targeting human cancer antigen STEAP1 with antibodies. bioRxiv.

Figure 3 PC3-STEAP-1 cells were transfected with a control siRNA (gray histogram) or a STEAP-1 siRNA (black histogram).

Cells were harvested 72 h later, stained with either isotype control mAb (solid histogram) or mAb 120.545 (open histograms), and then analyzed by FACS.

Challita-Eid, P. M., Morrison, K., Etessami, S., An, Z., Morrison, K. J., Perez-Villar, J. J., ... & Jakobovits, A. (2007). Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo. Cancer research, 67(12), 5798-5805.

Figure 4 Flow cytometry

3T3-neo, 3T3-STEAP-1, and LNCaP cells were stained with either mAb 92.30 or mAb 120.545 for 1 h at 4°C followed by phycoerythrin-conjugated antimouse IgG antibody. The cells were then analyzed by flow cytometry.

Challita-Eid, P. M., Morrison, K., Etessami, S., An, Z., Morrison, K. J., Perez-Villar, J. J., ... & Jakobovits, A. (2007). Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo. Cancer research, 67(12), 5798-5805.

Figure 5 Immunoprecipitates were prepared from cell lysates of 3T3-neo or 3T3-STEAP-1 cells with either mAb 92.30 (left) or mAb 120.545 (right).

The immunoprecipitates were subjected to SDS-PAGE, blotted, and stained with sheep STEAP-1 polyclonal antibody.

Challita-Eid, P. M., Morrison, K., Etessami, S., An, Z., Morrison, K. J., Perez-Villar, J. J., ... & Jakobovits, A. (2007). Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo. Cancer research, 67(12), 5798-5805.

Figure 6 LAPC-9AD cells were injected s.c. into SCID mice.

Mice (n = 10) were treated with either PBS, control mouse mAb, or mAbs 92.30 and 120.545 at 500 μg/dose. Treatment was given by i.p. injection, twice weekly for 6 wks starting on the day of tumor injection. Tumor volume was measured at the indicated time points. Points, mean tumor volume (mm³); bars, SE. Statistical analysis using the Kruskal-Wallis test shows P < 0.05 for both mAb 120.545 and mAb 92.30 treatments compared with PBS and control mAb treatments.

Challita-Eid, P. M., Morrison, K., Etessami, S., An, Z., Morrison, K. J., Perez-Villar, J. J., ... & Jakobovits, A. (2007). Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo. Cancer research, 67(12), 5798-5805.

Figure 7 UM-UC-3 cells were injected s.c. into SCID mice.

Treatment was given by i.p. injection with 1 mg/dose twice weekly for a total of three doses starting on day 8 (arrow). Tumor volume was measured at the indicated time points. Points, mean tumor volume (mm³); bars, SE. Statistical analysis using the Dunnett test shows P < 0.01 for both mAb 120.545 and mAb 92.30 treatments compared with control treatment.

Challita-Eid, P. M., Morrison, K., Etessami, S., An, Z., Morrison, K. J., Perez-Villar, J. J., ... & Jakobovits, A. (2007). Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo. Cancer research, 67(12), 5798-5805.