Chicken Anti-T. stejnegeri venom proteins Recombinant Antibody (clone TSS1) (CAT#: VS-0323-CJ54)
This chicken anti-T. stejnegeri venom proteins antibody (clone TSS1) is a product that recognizes T. stejnegeri venom proteins.
Specific Inquiry
ELISA
Figure 1 Binding analysis of anti-TS scFvs to various venom proteins.
Venom proteins collected from snakes DA, BM, TS, TM, NNA, and DRF were coated on ELISA wells and incubated with the individual, purified scFvs (5 μg/mL).
Inhib
Figure 2 Binding analysis of anti-TS scFvs using competitive ELISA.
Only 6.25 μg/mL of free TS proteins were needed to achieve more than 50% suppression on binding activities of TSL1 (57%) and TSL7 (57%). 12.5, 12.5, 25, and 100 μg/mL of TS proteins were needed to achieve the same inhibitory effects on binding activities of TSS1 (69%), TSS13 (68%), TSL2 (82%) and TSL8 (67%).
IA
Figure 4 Calculated dissociation constant (Kd) values of anti-TS antibodies.
Neut
Figure 5 Neutralization analysis of anti-TS scFvs in mice.
Polyclonal IgY antibodies from pre-immunized (Pre-IgY) or immunized (Imm-IgY) hens, equine-derived antivenom (4 mg), or a combination of six anti-TS scFvs (1 and 4 mg) were individually incubated with TS venom proteins at 37 °C for 1 h. These preparations were injected into mice, which were recorded hourly for 36 h.
Specifications
- Immunogen
- TS venom proteins from the bamboo vipers Trimeresurus stejnegeri (TS)
- Host Species
- Chicken
- Type
- Chicken IgY
- Specificity
- T. stejnegeri venom proteins
- Species Reactivity
- T. stejnegeri
- Clone
- TSS1
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Neutralization, Inhibition, Immunoassay
- Conjugate
- Unconjugated
- Related Disease
- Snakebite
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The recombinant scFv showed significant reactivity to TS venom proteins and a cross-reaction to Trimeresurus mucrosquamatus venom proteins.
ELISA: 1/200-1/2000
Western blot: 1 μg/mL-10 μg/mL
Inhibition assay: 0.25 mg-1 mg
Target
- Alternative Names
- T. stejnegeri venom proteins
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0323-CJ55 | Chicken Anti-T. stejnegeri venom proteins Recombinant Antibody (clone TSS13) | ELISA, WB, Neut, Inhib, IA | Chicken IgY |
| VS-0323-CJ56 | Chicken Anti-T. stejnegeri venom proteins Recombinant Antibody (clone TSL1) | ELISA, WB, Neut, Inhib, IA | Chicken IgY |
| VS-0323-CJ57 | Chicken Anti-T. stejnegeri venom proteins Recombinant Antibody (clone TSL2) | ELISA, WB, Neut, Inhib, IA | Chicken IgY |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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