Recombinant Camel Anti-GFLV Single Domain Antibody (HPAB-0144-YJ-VHH) (CAT#: HPAB-0144-YJ-VHH)
This product is a recombinant Camel single domain antibody that recognizes GFLV. It can be used in plant resistant to GFLV.
Specific Inquiry
Figure 1 Nb23 recognizes different GFLV isolates but not ArMV and remains functional upon fusion to GFP
Dynamic light scattering (DLS) analyses of GFLV -F13 alone (green curve) and GFLV complexed to Nb23 (red curve) or to Nb23:EGFP (blue curve).
Hemmer, C., Djennane, S., Ackerer, L., Hleibieh, K., Marmonier, A., Gersch, S., ... & Gertz, C. (2018). sdAb‐mediated resistance to Grapevine fanleaf virus in plants. Plant biotechnology journal, 16(2), 660-671.
ELISA
Figure 2 N. benthamiana infected with GFLV-GHu, GFLV-CP+3 or GFLV-Tyr216His were tested by DAS-ELISA with either conventional antibodies or Nb23 for detection.
Conventional antibodies recognize all GFLV isolates contrarily to Nb23 that fails to detect GFLV-CP+3 and GFLV-Tyr216His, indicating reduced binding of Nb23 to GFLV EV.
Orlov, I., Hemmer, C., Ackerer, L., Lorber, B., Ghannam, A., Poignavent, V., ... & Hily, J. M. (2019). Structural basis of sdAb-recognition of grapevine fanleaf virus and of virus resistance loss. bioRxiv, 728907.
Specifications
- Immunogen
- Purified GFLV strain F13
- Host Species
- Camel
- Type
- Camel VHH
- Specificity
- GFLV
- Species Reactivity
- GFLV
- Clone
- HPAB-0144-YJ-VHH
- Applications
- ELISA, FuncS
- Related Disease
- Fanleaf disease
Applications
- Application Notes
- The antibody was validated for ELISA, Functional Assay. For details, refer to Published Data.
Target
- Alternative Names
- Grapevine fanleaf virus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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